Genetic analysis of the cell binding domain region of the chicken fibronectin gene

Shigeo Kubomura, Masanobu Obara, Yuji Karasaki, Hatsumi Taniguchi, Sadao Gotoh, Tohru Tsuda, Ken Higashi, Keiichi Ohsato, Hideyasu Hirano

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11 Citations (Scopus)

Abstract

We have determined the nucleotide sequence of the cell binding domain region of the chicken fibronectin gene and analyzed it evolutionaly. We present here the complete nucleotide sequence of 4.3 kb HindIII/EcoRI segment from the clone λFC23 of the chicken fibronectin gene. There were five exons in this segment. When we lined up the amino acid of exons 28, 29 and 31, three alignments, known as the Type III repeat, appeared. Tetrapeptide, -RGDS-, called the cell binding domain, existed in the second repeat, coding exon 30. It was presumed that the Type III repeats were composed of two exons in the chicken gene, the same as in the rat and humans. We found repeatedly appearing amino-acid sequences such as -TIT- (three arrays in these Type III repeats) but also found one of the amino acids substituted in the tripeptide in these Type III repeats (seven arrays). We analyzed these repeats from the point of view of evolution. We used three of the nucleotide sequences (12-18 bp) coding such -TIT- repeats as a unit length for comparing the various homologies after dividing the coding region into 56 segments. The mutual homology of the divided segments to each one of three showed 53% on average. On the other hand, the mutual nucleotide homology of the Type III repeat was 44%. This suggested that the Type III repeat may have been developed by frequent duplication of small gene units.

Original languageEnglish
Pages (from-to)171-181
Number of pages11
JournalBBA - Gene Structure and Expression
Volume910
Issue number2
DOIs
Publication statusPublished - Nov 20 1987
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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