Genetic analysis of the gene cluster responsible for synthesis of serotype e-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans

Yasuo Yoshida, Yoshio Nakano, Nao Suzuki, Hiroshi Nakao, Yoshihisa Yamashita, Toshihiko Koga

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A gene cluster associated with the biosynthesis of the serotype e-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans IDH1705 belonging to serotype e was cloned and sequenced. This cluster consisted of 18 open reading frames. Escherichia coli produced the polysaccharide that reacts with the serotype e-specific antiserum when transformed with a plasmid containing the cluster. Comparing the structure of the gene cluster with similar clusters from A. actinomycetemcomitans strains Y4 (serotype b) and NCTC9710 (serotype c) revealed that a 5.3-kb region containing the distal half of one gene and two entire genes in the cluster from strain IDH1705 replaced a 6.2-kb region containing eight genes in the cluster from strain Y4, and a 4.7-kb region containing four genes in the cluster from strain NCTC9710. These results suggest that this region is essential to the antigenic specificity of serotype e A. actinomycetemcomitans. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)457-461
Number of pages5
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1489
Issue number2-3
DOIs
Publication statusPublished - Dec 23 1999

Fingerprint

Aggregatibacter actinomycetemcomitans
Multigene Family
Polysaccharides
Genes
Antigens
Biosynthesis
Escherichia coli
Open Reading Frames
Serogroup
Immune Sera
Epitopes
Plasmids

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Cite this

Genetic analysis of the gene cluster responsible for synthesis of serotype e-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans. / Yoshida, Yasuo; Nakano, Yoshio; Suzuki, Nao; Nakao, Hiroshi; Yamashita, Yoshihisa; Koga, Toshihiko.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1489, No. 2-3, 23.12.1999, p. 457-461.

Research output: Contribution to journalArticle

@article{74744cbc2ceb48e5867489d8e7ebf4a9,
title = "Genetic analysis of the gene cluster responsible for synthesis of serotype e-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans",
abstract = "A gene cluster associated with the biosynthesis of the serotype e-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans IDH1705 belonging to serotype e was cloned and sequenced. This cluster consisted of 18 open reading frames. Escherichia coli produced the polysaccharide that reacts with the serotype e-specific antiserum when transformed with a plasmid containing the cluster. Comparing the structure of the gene cluster with similar clusters from A. actinomycetemcomitans strains Y4 (serotype b) and NCTC9710 (serotype c) revealed that a 5.3-kb region containing the distal half of one gene and two entire genes in the cluster from strain IDH1705 replaced a 6.2-kb region containing eight genes in the cluster from strain Y4, and a 4.7-kb region containing four genes in the cluster from strain NCTC9710. These results suggest that this region is essential to the antigenic specificity of serotype e A. actinomycetemcomitans. Copyright (C) 1999 Elsevier Science B.V.",
author = "Yasuo Yoshida and Yoshio Nakano and Nao Suzuki and Hiroshi Nakao and Yoshihisa Yamashita and Toshihiko Koga",
year = "1999",
month = "12",
day = "23",
doi = "10.1016/S0167-4781(99)00192-X",
language = "English",
volume = "1489",
pages = "457--461",
journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier BV",
number = "2-3",

}

TY - JOUR

T1 - Genetic analysis of the gene cluster responsible for synthesis of serotype e-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans

AU - Yoshida, Yasuo

AU - Nakano, Yoshio

AU - Suzuki, Nao

AU - Nakao, Hiroshi

AU - Yamashita, Yoshihisa

AU - Koga, Toshihiko

PY - 1999/12/23

Y1 - 1999/12/23

N2 - A gene cluster associated with the biosynthesis of the serotype e-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans IDH1705 belonging to serotype e was cloned and sequenced. This cluster consisted of 18 open reading frames. Escherichia coli produced the polysaccharide that reacts with the serotype e-specific antiserum when transformed with a plasmid containing the cluster. Comparing the structure of the gene cluster with similar clusters from A. actinomycetemcomitans strains Y4 (serotype b) and NCTC9710 (serotype c) revealed that a 5.3-kb region containing the distal half of one gene and two entire genes in the cluster from strain IDH1705 replaced a 6.2-kb region containing eight genes in the cluster from strain Y4, and a 4.7-kb region containing four genes in the cluster from strain NCTC9710. These results suggest that this region is essential to the antigenic specificity of serotype e A. actinomycetemcomitans. Copyright (C) 1999 Elsevier Science B.V.

AB - A gene cluster associated with the biosynthesis of the serotype e-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans IDH1705 belonging to serotype e was cloned and sequenced. This cluster consisted of 18 open reading frames. Escherichia coli produced the polysaccharide that reacts with the serotype e-specific antiserum when transformed with a plasmid containing the cluster. Comparing the structure of the gene cluster with similar clusters from A. actinomycetemcomitans strains Y4 (serotype b) and NCTC9710 (serotype c) revealed that a 5.3-kb region containing the distal half of one gene and two entire genes in the cluster from strain IDH1705 replaced a 6.2-kb region containing eight genes in the cluster from strain Y4, and a 4.7-kb region containing four genes in the cluster from strain NCTC9710. These results suggest that this region is essential to the antigenic specificity of serotype e A. actinomycetemcomitans. Copyright (C) 1999 Elsevier Science B.V.

UR - http://www.scopus.com/inward/record.url?scp=0032758724&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032758724&partnerID=8YFLogxK

U2 - 10.1016/S0167-4781(99)00192-X

DO - 10.1016/S0167-4781(99)00192-X

M3 - Article

C2 - 10673051

AN - SCOPUS:0032758724

VL - 1489

SP - 457

EP - 461

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 2-3

ER -