Genetic analysis of the gene cluster responsible for synthesis of serotype e-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans

Yasuo Yoshida, Yoshio Nakano, Nao Suzuki, Hiroshi Nakao, Yoshihisa Yamashita, Toshihiko Koga

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15 Citations (Scopus)

Abstract

A gene cluster associated with the biosynthesis of the serotype e-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans IDH1705 belonging to serotype e was cloned and sequenced. This cluster consisted of 18 open reading frames. Escherichia coli produced the polysaccharide that reacts with the serotype e-specific antiserum when transformed with a plasmid containing the cluster. Comparing the structure of the gene cluster with similar clusters from A. actinomycetemcomitans strains Y4 (serotype b) and NCTC9710 (serotype c) revealed that a 5.3-kb region containing the distal half of one gene and two entire genes in the cluster from strain IDH1705 replaced a 6.2-kb region containing eight genes in the cluster from strain Y4, and a 4.7-kb region containing four genes in the cluster from strain NCTC9710. These results suggest that this region is essential to the antigenic specificity of serotype e A. actinomycetemcomitans. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)457-461
Number of pages5
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1489
Issue number2-3
DOIs
Publication statusPublished - Dec 23 1999

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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