Genetic and alkaloid analysis of Papaver species and their F1 hybrid by RAPD, HPLC and ELISA

Yukihiro Shoyama, Fumiko Kawachi, Hiroyuki Tanaka, Rie Nakai, Toshiro Shibata, Kozaburo Nishi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum Lindle and P. pseudo-orientale Medw. Analysis of random-amplified polymorphic DNA (RAPD) using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.

Original languageEnglish
Pages (from-to)207-217
Number of pages11
JournalForensic Science International
Volume91
Issue number3
DOIs
Publication statusPublished - Feb 16 1998

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Papaver
Alkaloids
Enzyme-Linked Immunosorbent Assay
High Pressure Liquid Chromatography
Thebaine
DNA
Oligonucleotides
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Genetic and alkaloid analysis of Papaver species and their F1 hybrid by RAPD, HPLC and ELISA. / Shoyama, Yukihiro; Kawachi, Fumiko; Tanaka, Hiroyuki; Nakai, Rie; Shibata, Toshiro; Nishi, Kozaburo.

In: Forensic Science International, Vol. 91, No. 3, 16.02.1998, p. 207-217.

Research output: Contribution to journalArticle

Shoyama, Yukihiro ; Kawachi, Fumiko ; Tanaka, Hiroyuki ; Nakai, Rie ; Shibata, Toshiro ; Nishi, Kozaburo. / Genetic and alkaloid analysis of Papaver species and their F1 hybrid by RAPD, HPLC and ELISA. In: Forensic Science International. 1998 ; Vol. 91, No. 3. pp. 207-217.
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