Genetic and chemical analysis of deep purple flower in soybean

Ryoji Takahashi, Fan Yan, Shaokang Di, Yoshinori Murai, Tsukasa Iwashina, Toyoaki Anai

Research output: Contribution to journalArticlepeer-review

Abstract

A soybean [Glycine max (L.) Merr.] mutant linproducing deep purple flowers (E013-C-1) wadeveloped from an ethylmethane sulfonate-treated population of the cultivar ‘Bay’, whichas purple flowers. Genetic analysis was performed on a cross between ‘E013-C-1’ anthe cultivar ‘Clark’ that had purple flowers. Fplants had purple flowers whereas F2 plantsegregated into a 3:1 purple/deep purple ratioThe results suggest that a single gene controls flower color and that purple color is dominant to deep purple. F3 plants derived from F2 plants with deep purple flowers were fixed for deep purple flowers. F3 plants derived from F2 plants with purple flowers segregated into two families segregating for flower color and one family that was fixed for purple flower color. The results confirmed that a single gene controls flower color and its recessive allele is responsible for deep purple flower color. The gene was designated Wd. Linkage mapping with simple sequence repeat (SSR) markers suggested that the Wd gene was located between Satt612 and Sct_199 in chromosome 18. Deep purple petals contained 50% higher levels of anthocyanins than purple petals. The vacuolar pH of deep purple flowers was similar to that of purple ones. These results suggest that the Wd gene controls the amount of anthocyanins and it is responsible for the development of deep purple flowers.

Original languageEnglish
Pages (from-to)1893-1897
Number of pages5
JournalCrop Science
Volume57
Issue number4
DOIs
Publication statusPublished - 2017
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science

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