Genetic and functional interaction between Ryh1 and Ypt3

Two Rab GTPases that function in S. pombe secretory pathway

Yi He, Reiko Sugiura, Yan Ma, Ayako Kita, Lu Deng, Kaoru Takegawa, Ken Matsuoka, Hisato Shuntoh, Takayoshi Kuno

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We have previously isolated ypt3-i5 mutant and showed that Ypt3 GTPase functions in the fission yeast secretory pathway. Here, the same genetic screen led to the isolation of ryh1-i6, a mutant allele of the ryh1+ gene encoding a homolog of Rab6. The ryh1-i6 mutant showed phenotypes that support its role in retrograde traffic from endosome to the Golgi. Interestingly, ryh1+ gene deletion was synthetically lethal with ypt3-i5 mutation. Consistently, the over-expression of the GDP-conformational mutant, Ryh1T25 N, inhibited the growth of ypt3-i5 mutant but had no effect on that of wild-type cells. Furthermore, the over-expression of the Ryh1T25N mutant inhibited the acid phosphatase glycosylation and exacerbated the cell wall integrity of ypt3-i5 mutant, but had no effect on those of wild-type cells. GFP-Ryh1 and GFP-Ypt3 both localized at the Golgi/endosome, but showed distinct subcellular localizations. The localization of GFP-Ryh1 in ypt3-i5 mutant and that of GFP-Ypt3 in ryh1-i6 mutant were distinct from those in wild-type cells. In addition, Ryh1 as well as Ypt3 were shown to be involved in acid phosphatase secretion. These results suggest that Ryh1 is involved in the secretory pathway and may have a potential overlapping function with Ypt3 in addition to its role in recycling.

Original languageEnglish
Pages (from-to)207-221
Number of pages15
JournalGenes to Cells
Volume11
Issue number3
DOIs
Publication statusPublished - Mar 1 2006
Externally publishedYes

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rab GTP-Binding Proteins
Schizosaccharomyces
Secretory Pathway
Endosomes
Acid Phosphatase
GTP Phosphohydrolases
Gene Deletion
Recycling
Glycosylation
Cell Wall
Alleles
Phenotype
Mutation
Growth
Genes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Genetic and functional interaction between Ryh1 and Ypt3 : Two Rab GTPases that function in S. pombe secretory pathway. / He, Yi; Sugiura, Reiko; Ma, Yan; Kita, Ayako; Deng, Lu; Takegawa, Kaoru; Matsuoka, Ken; Shuntoh, Hisato; Kuno, Takayoshi.

In: Genes to Cells, Vol. 11, No. 3, 01.03.2006, p. 207-221.

Research output: Contribution to journalArticle

He, Yi ; Sugiura, Reiko ; Ma, Yan ; Kita, Ayako ; Deng, Lu ; Takegawa, Kaoru ; Matsuoka, Ken ; Shuntoh, Hisato ; Kuno, Takayoshi. / Genetic and functional interaction between Ryh1 and Ypt3 : Two Rab GTPases that function in S. pombe secretory pathway. In: Genes to Cells. 2006 ; Vol. 11, No. 3. pp. 207-221.
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AU - Deng, Lu

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