Genetic basis of multiple resistance to the brown planthopper (Nilaparvata lugens stål) and the green rice leafhopper (nephotettix cincticeps uhler) in the rice cultivar ‘ASD7’ (oryza sativa L. ssp. indica)

Tan Van Mai, Daisuke Fujita, Masaya Matsumura, Atsushi Yoshimura, Hideshi Yasui

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The rice cultivar ASD7 (Oryza sativa L. ssp. indica) is resistant to the brown planthopper (BPH; Nilaparvata lugens Stål) and the green leafhopper (Nephotettix virescens Distant). Here, we analyzed multiple genetic resistance to BPH and the green rice leafhopper (GRH; Nephotettix cincticeps Uhler). Using two independent F2 populations derived from a cross between ASD7 and Taichung 65 (Oryza sativa ssp. japonica), we detected two QTLs (qBPH6 and qBPH12) for resistance to BPH and one QTL (qGRH5) for resistance to GRH. Linkage analysis in BC2F3 populations revealed that qBPH12 controlled resistance to BPH and co-segregated with SSR markers RM28466 and RM7376 in plants homozygous for the ASD7 allele at qBPH6 Plants homozygous for the ASD7 alleles at both QTLs showed a much faster antibiosis response to BPH than plants homozygous at only one of these QTLs. It revealed that epistatic interaction between qBPH6 and qBPH12 is the basis of resistance to BPH in ASD7 In addition, qGRH5 controlled resistance to GRH and co-segregated with SSR markers RM6082 and RM3381. qGRH5 is identical to GRH1 Thus, we clarified the genetic basis of multiple resistance of ASD7 to BPH and GRH.

Original languageEnglish
Pages (from-to)420-429
Number of pages10
JournalBreeding Science
Volume65
Issue number5
DOIs
Publication statusPublished - Dec 19 2015

Fingerprint

Nephotettix cincticeps
Nephotettix nigropictus
Nilaparvata lugens
Hemiptera
quantitative trait loci
Oryza sativa
rice
cultivars
Nephotettix virescens
Alleles
Antibiosis
alleles
antibiosis
genetic resistance
Cicadellidae
Population
linkage (genetics)
Oryza

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Genetic basis of multiple resistance to the brown planthopper (Nilaparvata lugens stål) and the green rice leafhopper (nephotettix cincticeps uhler) in the rice cultivar ‘ASD7’ (oryza sativa L. ssp. indica). / Mai, Tan Van; Fujita, Daisuke; Matsumura, Masaya; Yoshimura, Atsushi; Yasui, Hideshi.

In: Breeding Science, Vol. 65, No. 5, 19.12.2015, p. 420-429.

Research output: Contribution to journalArticle

@article{576ec8fbaf9944ebb5d5ca71e71aa8d8,
title = "Genetic basis of multiple resistance to the brown planthopper (Nilaparvata lugens st{\aa}l) and the green rice leafhopper (nephotettix cincticeps uhler) in the rice cultivar ‘ASD7’ (oryza sativa L. ssp. indica)",
abstract = "The rice cultivar ASD7 (Oryza sativa L. ssp. indica) is resistant to the brown planthopper (BPH; Nilaparvata lugens St{\aa}l) and the green leafhopper (Nephotettix virescens Distant). Here, we analyzed multiple genetic resistance to BPH and the green rice leafhopper (GRH; Nephotettix cincticeps Uhler). Using two independent F2 populations derived from a cross between ASD7 and Taichung 65 (Oryza sativa ssp. japonica), we detected two QTLs (qBPH6 and qBPH12) for resistance to BPH and one QTL (qGRH5) for resistance to GRH. Linkage analysis in BC2F3 populations revealed that qBPH12 controlled resistance to BPH and co-segregated with SSR markers RM28466 and RM7376 in plants homozygous for the ASD7 allele at qBPH6 Plants homozygous for the ASD7 alleles at both QTLs showed a much faster antibiosis response to BPH than plants homozygous at only one of these QTLs. It revealed that epistatic interaction between qBPH6 and qBPH12 is the basis of resistance to BPH in ASD7 In addition, qGRH5 controlled resistance to GRH and co-segregated with SSR markers RM6082 and RM3381. qGRH5 is identical to GRH1 Thus, we clarified the genetic basis of multiple resistance of ASD7 to BPH and GRH.",
author = "Mai, {Tan Van} and Daisuke Fujita and Masaya Matsumura and Atsushi Yoshimura and Hideshi Yasui",
year = "2015",
month = "12",
day = "19",
doi = "10.1270/jsbbs.65.420",
language = "English",
volume = "65",
pages = "420--429",
journal = "Breeding Science",
issn = "1344-7610",
publisher = "Japanese Society of Breeding",
number = "5",

}

TY - JOUR

T1 - Genetic basis of multiple resistance to the brown planthopper (Nilaparvata lugens stål) and the green rice leafhopper (nephotettix cincticeps uhler) in the rice cultivar ‘ASD7’ (oryza sativa L. ssp. indica)

AU - Mai, Tan Van

AU - Fujita, Daisuke

AU - Matsumura, Masaya

AU - Yoshimura, Atsushi

AU - Yasui, Hideshi

PY - 2015/12/19

Y1 - 2015/12/19

N2 - The rice cultivar ASD7 (Oryza sativa L. ssp. indica) is resistant to the brown planthopper (BPH; Nilaparvata lugens Stål) and the green leafhopper (Nephotettix virescens Distant). Here, we analyzed multiple genetic resistance to BPH and the green rice leafhopper (GRH; Nephotettix cincticeps Uhler). Using two independent F2 populations derived from a cross between ASD7 and Taichung 65 (Oryza sativa ssp. japonica), we detected two QTLs (qBPH6 and qBPH12) for resistance to BPH and one QTL (qGRH5) for resistance to GRH. Linkage analysis in BC2F3 populations revealed that qBPH12 controlled resistance to BPH and co-segregated with SSR markers RM28466 and RM7376 in plants homozygous for the ASD7 allele at qBPH6 Plants homozygous for the ASD7 alleles at both QTLs showed a much faster antibiosis response to BPH than plants homozygous at only one of these QTLs. It revealed that epistatic interaction between qBPH6 and qBPH12 is the basis of resistance to BPH in ASD7 In addition, qGRH5 controlled resistance to GRH and co-segregated with SSR markers RM6082 and RM3381. qGRH5 is identical to GRH1 Thus, we clarified the genetic basis of multiple resistance of ASD7 to BPH and GRH.

AB - The rice cultivar ASD7 (Oryza sativa L. ssp. indica) is resistant to the brown planthopper (BPH; Nilaparvata lugens Stål) and the green leafhopper (Nephotettix virescens Distant). Here, we analyzed multiple genetic resistance to BPH and the green rice leafhopper (GRH; Nephotettix cincticeps Uhler). Using two independent F2 populations derived from a cross between ASD7 and Taichung 65 (Oryza sativa ssp. japonica), we detected two QTLs (qBPH6 and qBPH12) for resistance to BPH and one QTL (qGRH5) for resistance to GRH. Linkage analysis in BC2F3 populations revealed that qBPH12 controlled resistance to BPH and co-segregated with SSR markers RM28466 and RM7376 in plants homozygous for the ASD7 allele at qBPH6 Plants homozygous for the ASD7 alleles at both QTLs showed a much faster antibiosis response to BPH than plants homozygous at only one of these QTLs. It revealed that epistatic interaction between qBPH6 and qBPH12 is the basis of resistance to BPH in ASD7 In addition, qGRH5 controlled resistance to GRH and co-segregated with SSR markers RM6082 and RM3381. qGRH5 is identical to GRH1 Thus, we clarified the genetic basis of multiple resistance of ASD7 to BPH and GRH.

UR - http://www.scopus.com/inward/record.url?scp=84950277670&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84950277670&partnerID=8YFLogxK

U2 - 10.1270/jsbbs.65.420

DO - 10.1270/jsbbs.65.420

M3 - Article

AN - SCOPUS:84950277670

VL - 65

SP - 420

EP - 429

JO - Breeding Science

JF - Breeding Science

SN - 1344-7610

IS - 5

ER -