Genetic characterization and fine mapping of S25, a hybrid male sterility gene, on rice chromosome 12

Takahiko Kubo, Atsushi Yoshimura, Nori Kurata

Research output: Contribution to journalArticle

Abstract

Hybrid male sterility genes are important factors in creating postzygotic reproductive isolation barriers in plants. One such gene, S25, is known to cause severe transmission ratio distortion in inter-subspecific progeny of cultivated rice Oryza sativa ssp. indica and japonica. To further characterize the S25 gene, we fine-mapped and genetically characterized the S25 gene using near-isogenic lines with reciprocal genetic backgrounds. We mapped the S25 locus within the 0.67–1.02 Mb region on rice chromosome 12. Further genetic analyses revealed that S25 substantially reduced male fertility in the japonica background, but not in the indica background. In first-generation hybrid progeny, S25 had a milder effect than it had in the japonica background. These results suggest that the expression of S25 is epistatically regulated by at least one partially dominant gene present in the indica genome. This finding supports our previous studies showing that hybrid male sterility due to pollen killer genes results from epistatic interaction with other genes that are hidden in the genetic background.

Original languageEnglish
Pages (from-to)205-212
Number of pages8
JournalGenes and Genetic Systems
Volume92
Issue number4
DOIs
Publication statusPublished - Jan 1 2017

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Chromosomes, Human, Pair 12
Male Infertility
Genes
Genetic Epistasis
Reproductive Isolation
Dominant Genes
Pollen
Fertility
Oryza
Genome

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Genetic characterization and fine mapping of S25, a hybrid male sterility gene, on rice chromosome 12. / Kubo, Takahiko; Yoshimura, Atsushi; Kurata, Nori.

In: Genes and Genetic Systems, Vol. 92, No. 4, 01.01.2017, p. 205-212.

Research output: Contribution to journalArticle

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