Genetic characterization of the rice sheath blight pathogen Rhizoctonia solani AG1-IA in North Vietnam by rep-pcr and sequence analysis

Matsumoto Masaru, H. V. Cuong

Research output: Contribution to journalArticle

Abstract

Representative isolates of Rhizoctonia solani AG1-IA associated with rice sheath blight obtained from North Vietnam were characterized using rep-PCR fingerprinting and ITS-5.8S rDNA sequencing. For rep-PCR assays primers ERIC2 and BOXA1R were used. Sequences of these isolates were aligned with other known R. solani sequences from the GenBank. Rep-PCR analysis revealed that two genotypes (Rep-Vet1 and Rep-Vet2) were present based on the fingerprinting dendrograms having the similarity coefficient score at 75% differentiation. The two clonal populations were scattered and had different geographical localizations in North Vietnam.

Original languageEnglish
Pages (from-to)377-380
Number of pages4
JournalJournal of Plant Pathology
Volume96
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Thanatephorus cucumeris
blight
Vietnam
sequence analysis
rice
pathogens
genotype
assays

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

@article{6ae6344a345a4800b7f2670f0c8405f2,
title = "Genetic characterization of the rice sheath blight pathogen Rhizoctonia solani AG1-IA in North Vietnam by rep-pcr and sequence analysis",
abstract = "Representative isolates of Rhizoctonia solani AG1-IA associated with rice sheath blight obtained from North Vietnam were characterized using rep-PCR fingerprinting and ITS-5.8S rDNA sequencing. For rep-PCR assays primers ERIC2 and BOXA1R were used. Sequences of these isolates were aligned with other known R. solani sequences from the GenBank. Rep-PCR analysis revealed that two genotypes (Rep-Vet1 and Rep-Vet2) were present based on the fingerprinting dendrograms having the similarity coefficient score at 75{\%} differentiation. The two clonal populations were scattered and had different geographical localizations in North Vietnam.",
author = "Matsumoto Masaru and Cuong, {H. V.}",
year = "2014",
doi = "10.4454/JPP.V96I2.018",
language = "English",
volume = "96",
pages = "377--380",
journal = "Journal of Plant Pathology",
issn = "1125-4653",
publisher = "Edizioni E.T.S.",
number = "2",

}

TY - JOUR

T1 - Genetic characterization of the rice sheath blight pathogen Rhizoctonia solani AG1-IA in North Vietnam by rep-pcr and sequence analysis

AU - Masaru, Matsumoto

AU - Cuong, H. V.

PY - 2014

Y1 - 2014

N2 - Representative isolates of Rhizoctonia solani AG1-IA associated with rice sheath blight obtained from North Vietnam were characterized using rep-PCR fingerprinting and ITS-5.8S rDNA sequencing. For rep-PCR assays primers ERIC2 and BOXA1R were used. Sequences of these isolates were aligned with other known R. solani sequences from the GenBank. Rep-PCR analysis revealed that two genotypes (Rep-Vet1 and Rep-Vet2) were present based on the fingerprinting dendrograms having the similarity coefficient score at 75% differentiation. The two clonal populations were scattered and had different geographical localizations in North Vietnam.

AB - Representative isolates of Rhizoctonia solani AG1-IA associated with rice sheath blight obtained from North Vietnam were characterized using rep-PCR fingerprinting and ITS-5.8S rDNA sequencing. For rep-PCR assays primers ERIC2 and BOXA1R were used. Sequences of these isolates were aligned with other known R. solani sequences from the GenBank. Rep-PCR analysis revealed that two genotypes (Rep-Vet1 and Rep-Vet2) were present based on the fingerprinting dendrograms having the similarity coefficient score at 75% differentiation. The two clonal populations were scattered and had different geographical localizations in North Vietnam.

UR - http://www.scopus.com/inward/record.url?scp=84904004039&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904004039&partnerID=8YFLogxK

U2 - 10.4454/JPP.V96I2.018

DO - 10.4454/JPP.V96I2.018

M3 - Article

AN - SCOPUS:84904004039

VL - 96

SP - 377

EP - 380

JO - Journal of Plant Pathology

JF - Journal of Plant Pathology

SN - 1125-4653

IS - 2

ER -