TY - JOUR
T1 - Genetic diagnosis of micrometastasis based on SCC antigen mRNA in cervical lymph nodes of head and neck cancer
AU - Hamakawa, Hiroyuki
AU - Fukizumi, Masakuni
AU - Yang, Bao
AU - Sumida, Tomoki
AU - Onishi, Akiko
AU - Tanioka, Hiroaki
AU - Sato, Hidemitsu
AU - Yumoto, Eiji
N1 - Funding Information:
This research was supported in part by the Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Scientific Research (B), 10470434, 1998.
PY - 1999
Y1 - 1999
N2 - This study is designed to assess gene expression of squamous cell carcinoma antigen (SCCA) mRNA to detect micrometasrases in cervical lymph nodes (LNs) of head and neck cancer. We examined the expression of SCCA mRNA in 12 primary tumors and 212 cervical LNs (101 LNs taken from 8 patients with tongue cancer, 71 from 7 patients with gingival cancer, 19 from 2 patients with laryngeal cancer, 9 from 2 patients with pharyngeal cancer, 7 from 1 patient with cancer of the buccal mucosa, and 5 from 1 patient with cancer of floor of the mouth). Detectability of metastatic LNs by nested and single reverse transcriptase-polymerase chain reaction (RT-PCR) was compared with semiserial sections (hematoxylin-eosin staining and keratin immunostaining). All primary tumors expressed SCCA mRNA. Of 198 histologically metastasis- negative nodes, SCCA mRNA was detected in 37 (18.7%) by nested PCR. Eleven micrometastatic foci in 9 LNs (4.6%) were discovered by semiserial sectioning. This suggests that SCCA mRNA is a promising tumor marker for detecting the micrometastases in cervical LNs of head and neck cancer.
AB - This study is designed to assess gene expression of squamous cell carcinoma antigen (SCCA) mRNA to detect micrometasrases in cervical lymph nodes (LNs) of head and neck cancer. We examined the expression of SCCA mRNA in 12 primary tumors and 212 cervical LNs (101 LNs taken from 8 patients with tongue cancer, 71 from 7 patients with gingival cancer, 19 from 2 patients with laryngeal cancer, 9 from 2 patients with pharyngeal cancer, 7 from 1 patient with cancer of the buccal mucosa, and 5 from 1 patient with cancer of floor of the mouth). Detectability of metastatic LNs by nested and single reverse transcriptase-polymerase chain reaction (RT-PCR) was compared with semiserial sections (hematoxylin-eosin staining and keratin immunostaining). All primary tumors expressed SCCA mRNA. Of 198 histologically metastasis- negative nodes, SCCA mRNA was detected in 37 (18.7%) by nested PCR. Eleven micrometastatic foci in 9 LNs (4.6%) were discovered by semiserial sectioning. This suggests that SCCA mRNA is a promising tumor marker for detecting the micrometastases in cervical LNs of head and neck cancer.
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U2 - 10.1023/A:1006732911057
DO - 10.1023/A:1006732911057
M3 - Article
C2 - 10845558
AN - SCOPUS:0033505688
SN - 0262-0898
VL - 17
SP - 593
EP - 599
JO - Clinical and Experimental Metastasis
JF - Clinical and Experimental Metastasis
IS - 7
ER -
