Genetic diagnosis of micrometastasis based on SCC antigen mRNA in cervical lymph nodes of head and neck cancer

Hiroyuki Hamakawa, Masakuni Fukizumi, Bao Yang, Tomoki Sumida, Akiko Onishi, Hiroaki Tanioka, Hidemitsu Sato, Eiji Yumoto

Research output: Contribution to journalArticle

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Abstract

This study is designed to assess gene expression of squamous cell carcinoma antigen (SCCA) mRNA to detect micrometasrases in cervical lymph nodes (LNs) of head and neck cancer. We examined the expression of SCCA mRNA in 12 primary tumors and 212 cervical LNs (101 LNs taken from 8 patients with tongue cancer, 71 from 7 patients with gingival cancer, 19 from 2 patients with laryngeal cancer, 9 from 2 patients with pharyngeal cancer, 7 from 1 patient with cancer of the buccal mucosa, and 5 from 1 patient with cancer of floor of the mouth). Detectability of metastatic LNs by nested and single reverse transcriptase-polymerase chain reaction (RT-PCR) was compared with semiserial sections (hematoxylin-eosin staining and keratin immunostaining). All primary tumors expressed SCCA mRNA. Of 198 histologically metastasis- negative nodes, SCCA mRNA was detected in 37 (18.7%) by nested PCR. Eleven micrometastatic foci in 9 LNs (4.6%) were discovered by semiserial sectioning. This suggests that SCCA mRNA is a promising tumor marker for detecting the micrometastases in cervical LNs of head and neck cancer.

Original languageEnglish
Pages (from-to)593-599
Number of pages7
JournalClinical and Experimental Metastasis
Volume17
Issue number7
DOIs
Publication statusPublished - Dec 1 1999

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Neoplasm Micrometastasis
Head and Neck Neoplasms
Lymph Nodes
Messenger RNA
Neoplasms
Pharyngeal Neoplasms
Tongue Neoplasms
Mouth Floor
Laryngeal Neoplasms
Mouth Neoplasms
Mouth Mucosa
Hematoxylin
Eosine Yellowish-(YS)
Tumor Biomarkers
Keratins
Reverse Transcriptase Polymerase Chain Reaction
squamous cell carcinoma-related antigen
Staining and Labeling
Neoplasm Metastasis
Gene Expression

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Genetic diagnosis of micrometastasis based on SCC antigen mRNA in cervical lymph nodes of head and neck cancer. / Hamakawa, Hiroyuki; Fukizumi, Masakuni; Yang, Bao; Sumida, Tomoki; Onishi, Akiko; Tanioka, Hiroaki; Sato, Hidemitsu; Yumoto, Eiji.

In: Clinical and Experimental Metastasis, Vol. 17, No. 7, 01.12.1999, p. 593-599.

Research output: Contribution to journalArticle

Hamakawa, Hiroyuki ; Fukizumi, Masakuni ; Yang, Bao ; Sumida, Tomoki ; Onishi, Akiko ; Tanioka, Hiroaki ; Sato, Hidemitsu ; Yumoto, Eiji. / Genetic diagnosis of micrometastasis based on SCC antigen mRNA in cervical lymph nodes of head and neck cancer. In: Clinical and Experimental Metastasis. 1999 ; Vol. 17, No. 7. pp. 593-599.
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