Genetic diversity and population structure of Plasmodium falciparum in the Philippines

Moritoshi Iwagami, Pilarita T. Rivera, Elena A. Villacorte, Aleyla D. Escueta, Toshimitsu Hatabu, Shin Ichiro Kawazu, Toshiyuki Hayakawa, Kazuyuki Tanabe, Shigeyuki Kano

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Background. In the Philippines, malaria morbidity and mortality have decreased since the 1990s by effective malaria control. Several epidemiological surveys have been performed in the country, but the characteristics of the Plasmodium falciparum populations are not yet fully understood. In this study, the genetic structure of P. falciparum populations in the Philippines was examined. Methods. Population genetic analyses based on polymorphisms of 10 microsatellite loci of the parasite were conducted on 92 isolates from three provinces (Kalinga, Palawan, and Davao del Norte) with different malaria endemicity. Results. The levels of genetic diversity and the effective population sizes of P. falciparum in the Philippines were similar to those reported in the mainland of Southeast Asia or South America. In the low malaria transmission area (Kalinga), there was a low level of genetic diversity and a strong linkage disequilibrium (LD) when the single-clone haplotype (SCH) was used in the multilocus LD analysis, while in the high malaria transmission areas (Palawan and Davao del Norte), there was a high level of genetic diversity and a weak LD when SCH was used in the multilocus LD analysis. On the other hand, when the unique haplotypes were used in the multilocus LD analysis, no significant LD was observed in the Kalinga and the Palawan populations. The Kalinga and the Palawan populations were, therefore, estimated to have an epidemic population structure. The three populations were moderately differentiated from each other. Conclusion. In each area, the level of genetic diversity correlates with the local malaria endemicity. These findings confirm that population genetic analyses using microsatellite loci are a useful tool for evaluating malaria endemicity.

Original languageEnglish
Article number96
JournalMalaria Journal
Volume8
Issue number1
DOIs
Publication statusPublished - Jun 8 2009
Externally publishedYes

Fingerprint

Philippines
Plasmodium falciparum
Linkage Disequilibrium
Malaria
Population
Haplotypes
Population Genetics
Microsatellite Repeats
Clone Cells
Southeastern Asia
South America
Genetic Structures
Population Density
Parasites
Morbidity
Mortality

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Iwagami, M., Rivera, P. T., Villacorte, E. A., Escueta, A. D., Hatabu, T., Kawazu, S. I., ... Kano, S. (2009). Genetic diversity and population structure of Plasmodium falciparum in the Philippines. Malaria Journal, 8(1), [96]. https://doi.org/10.1186/1475-2875-8-96

Genetic diversity and population structure of Plasmodium falciparum in the Philippines. / Iwagami, Moritoshi; Rivera, Pilarita T.; Villacorte, Elena A.; Escueta, Aleyla D.; Hatabu, Toshimitsu; Kawazu, Shin Ichiro; Hayakawa, Toshiyuki; Tanabe, Kazuyuki; Kano, Shigeyuki.

In: Malaria Journal, Vol. 8, No. 1, 96, 08.06.2009.

Research output: Contribution to journalArticle

Iwagami, M, Rivera, PT, Villacorte, EA, Escueta, AD, Hatabu, T, Kawazu, SI, Hayakawa, T, Tanabe, K & Kano, S 2009, 'Genetic diversity and population structure of Plasmodium falciparum in the Philippines', Malaria Journal, vol. 8, no. 1, 96. https://doi.org/10.1186/1475-2875-8-96
Iwagami M, Rivera PT, Villacorte EA, Escueta AD, Hatabu T, Kawazu SI et al. Genetic diversity and population structure of Plasmodium falciparum in the Philippines. Malaria Journal. 2009 Jun 8;8(1). 96. https://doi.org/10.1186/1475-2875-8-96
Iwagami, Moritoshi ; Rivera, Pilarita T. ; Villacorte, Elena A. ; Escueta, Aleyla D. ; Hatabu, Toshimitsu ; Kawazu, Shin Ichiro ; Hayakawa, Toshiyuki ; Tanabe, Kazuyuki ; Kano, Shigeyuki. / Genetic diversity and population structure of Plasmodium falciparum in the Philippines. In: Malaria Journal. 2009 ; Vol. 8, No. 1.
@article{ef73de64acec488bad6002c5e62809ae,
title = "Genetic diversity and population structure of Plasmodium falciparum in the Philippines",
abstract = "Background. In the Philippines, malaria morbidity and mortality have decreased since the 1990s by effective malaria control. Several epidemiological surveys have been performed in the country, but the characteristics of the Plasmodium falciparum populations are not yet fully understood. In this study, the genetic structure of P. falciparum populations in the Philippines was examined. Methods. Population genetic analyses based on polymorphisms of 10 microsatellite loci of the parasite were conducted on 92 isolates from three provinces (Kalinga, Palawan, and Davao del Norte) with different malaria endemicity. Results. The levels of genetic diversity and the effective population sizes of P. falciparum in the Philippines were similar to those reported in the mainland of Southeast Asia or South America. In the low malaria transmission area (Kalinga), there was a low level of genetic diversity and a strong linkage disequilibrium (LD) when the single-clone haplotype (SCH) was used in the multilocus LD analysis, while in the high malaria transmission areas (Palawan and Davao del Norte), there was a high level of genetic diversity and a weak LD when SCH was used in the multilocus LD analysis. On the other hand, when the unique haplotypes were used in the multilocus LD analysis, no significant LD was observed in the Kalinga and the Palawan populations. The Kalinga and the Palawan populations were, therefore, estimated to have an epidemic population structure. The three populations were moderately differentiated from each other. Conclusion. In each area, the level of genetic diversity correlates with the local malaria endemicity. These findings confirm that population genetic analyses using microsatellite loci are a useful tool for evaluating malaria endemicity.",
author = "Moritoshi Iwagami and Rivera, {Pilarita T.} and Villacorte, {Elena A.} and Escueta, {Aleyla D.} and Toshimitsu Hatabu and Kawazu, {Shin Ichiro} and Toshiyuki Hayakawa and Kazuyuki Tanabe and Shigeyuki Kano",
year = "2009",
month = "6",
day = "8",
doi = "10.1186/1475-2875-8-96",
language = "English",
volume = "8",
journal = "Malaria Journal",
issn = "1475-2875",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Genetic diversity and population structure of Plasmodium falciparum in the Philippines

AU - Iwagami, Moritoshi

AU - Rivera, Pilarita T.

AU - Villacorte, Elena A.

AU - Escueta, Aleyla D.

AU - Hatabu, Toshimitsu

AU - Kawazu, Shin Ichiro

AU - Hayakawa, Toshiyuki

AU - Tanabe, Kazuyuki

AU - Kano, Shigeyuki

PY - 2009/6/8

Y1 - 2009/6/8

N2 - Background. In the Philippines, malaria morbidity and mortality have decreased since the 1990s by effective malaria control. Several epidemiological surveys have been performed in the country, but the characteristics of the Plasmodium falciparum populations are not yet fully understood. In this study, the genetic structure of P. falciparum populations in the Philippines was examined. Methods. Population genetic analyses based on polymorphisms of 10 microsatellite loci of the parasite were conducted on 92 isolates from three provinces (Kalinga, Palawan, and Davao del Norte) with different malaria endemicity. Results. The levels of genetic diversity and the effective population sizes of P. falciparum in the Philippines were similar to those reported in the mainland of Southeast Asia or South America. In the low malaria transmission area (Kalinga), there was a low level of genetic diversity and a strong linkage disequilibrium (LD) when the single-clone haplotype (SCH) was used in the multilocus LD analysis, while in the high malaria transmission areas (Palawan and Davao del Norte), there was a high level of genetic diversity and a weak LD when SCH was used in the multilocus LD analysis. On the other hand, when the unique haplotypes were used in the multilocus LD analysis, no significant LD was observed in the Kalinga and the Palawan populations. The Kalinga and the Palawan populations were, therefore, estimated to have an epidemic population structure. The three populations were moderately differentiated from each other. Conclusion. In each area, the level of genetic diversity correlates with the local malaria endemicity. These findings confirm that population genetic analyses using microsatellite loci are a useful tool for evaluating malaria endemicity.

AB - Background. In the Philippines, malaria morbidity and mortality have decreased since the 1990s by effective malaria control. Several epidemiological surveys have been performed in the country, but the characteristics of the Plasmodium falciparum populations are not yet fully understood. In this study, the genetic structure of P. falciparum populations in the Philippines was examined. Methods. Population genetic analyses based on polymorphisms of 10 microsatellite loci of the parasite were conducted on 92 isolates from three provinces (Kalinga, Palawan, and Davao del Norte) with different malaria endemicity. Results. The levels of genetic diversity and the effective population sizes of P. falciparum in the Philippines were similar to those reported in the mainland of Southeast Asia or South America. In the low malaria transmission area (Kalinga), there was a low level of genetic diversity and a strong linkage disequilibrium (LD) when the single-clone haplotype (SCH) was used in the multilocus LD analysis, while in the high malaria transmission areas (Palawan and Davao del Norte), there was a high level of genetic diversity and a weak LD when SCH was used in the multilocus LD analysis. On the other hand, when the unique haplotypes were used in the multilocus LD analysis, no significant LD was observed in the Kalinga and the Palawan populations. The Kalinga and the Palawan populations were, therefore, estimated to have an epidemic population structure. The three populations were moderately differentiated from each other. Conclusion. In each area, the level of genetic diversity correlates with the local malaria endemicity. These findings confirm that population genetic analyses using microsatellite loci are a useful tool for evaluating malaria endemicity.

UR - http://www.scopus.com/inward/record.url?scp=66249140986&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=66249140986&partnerID=8YFLogxK

U2 - 10.1186/1475-2875-8-96

DO - 10.1186/1475-2875-8-96

M3 - Article

C2 - 19422722

AN - SCOPUS:66249140986

VL - 8

JO - Malaria Journal

JF - Malaria Journal

SN - 1475-2875

IS - 1

M1 - 96

ER -