Genetic evidence for key roles of decorin and biglycan in dentin mineralization

Naoto Haruyama, Taduru L. Sreenath, Shigeki Suzuki, Xiaomei Yao, Zhigang Wang, Yong Wang, Cherlita Honeycutt, Renato V. Iozzo, Marian F. Young, Ashok B. Kulkarni

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Targeted disruption of the dentin sialophosphoprotein (DSPP) gene in the mice (Dspp-/-) results in dentin mineralization defects with enlarged predentin phenotype similar to human dentinogenesis imperfecta type III. Using DSPP/biglycan (Dspp-/-Bgn-/0) and DSPP/decorin (Dspp-/-Dcn-/-) double knockout mice, here we determined that the enlarged predentin layer in Dspp-/- teeth is rescued in the absence of decorin, but not in the absence of biglycan. However, Fourier transform infrared (FTIR) spectroscopy analysis reveals similar hypomineralization of dentin in both Dspp-/-Bgn-/0 and Dspp-/-Dcn-/- teeth. Atomic force microscopy (AFM) analysis of collagen fibrils in dentin shows subtle differences in the collagen fibril morphology in these genotypes. The reduction of enlarged predentin in Dspp-/-Dcn-/- mice suggests that the elevated level of decorin in Dspp-/- predentin interferes with the mineralization process at the dentin mineralization front. On the other hand, the lack of DSPP and biglycan leads to the increased number of calcospherites in Dspp-/-Bgn-/0 predentin, suggesting that a failure in coalescence of calcospherites was augmented in Dspp-/-Bgn-/0 teeth as compared to Dspp-/- teeth. These findings indicate that normal expression of small leucine rich proteoglycans, such as biglycan and decorin, plays an important role in the highly orchestrated process of dentin mineralization.

Original languageEnglish
Pages (from-to)129-136
Number of pages8
JournalMatrix Biology
Volume28
Issue number3
DOIs
Publication statusPublished - Apr 1 2009
Externally publishedYes

Fingerprint

Biglycan
Decorin
Dentin
Tooth
Dentinogenesis Imperfecta
Collagen
Atomic Force Microscopy
Fourier Transform Infrared Spectroscopy
Knockout Mice
Genotype
Phenotype
dentin sialophosphoprotein
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Haruyama, N., Sreenath, T. L., Suzuki, S., Yao, X., Wang, Z., Wang, Y., ... Kulkarni, A. B. (2009). Genetic evidence for key roles of decorin and biglycan in dentin mineralization. Matrix Biology, 28(3), 129-136. https://doi.org/10.1016/j.matbio.2009.01.005

Genetic evidence for key roles of decorin and biglycan in dentin mineralization. / Haruyama, Naoto; Sreenath, Taduru L.; Suzuki, Shigeki; Yao, Xiaomei; Wang, Zhigang; Wang, Yong; Honeycutt, Cherlita; Iozzo, Renato V.; Young, Marian F.; Kulkarni, Ashok B.

In: Matrix Biology, Vol. 28, No. 3, 01.04.2009, p. 129-136.

Research output: Contribution to journalArticle

Haruyama, N, Sreenath, TL, Suzuki, S, Yao, X, Wang, Z, Wang, Y, Honeycutt, C, Iozzo, RV, Young, MF & Kulkarni, AB 2009, 'Genetic evidence for key roles of decorin and biglycan in dentin mineralization', Matrix Biology, vol. 28, no. 3, pp. 129-136. https://doi.org/10.1016/j.matbio.2009.01.005
Haruyama, Naoto ; Sreenath, Taduru L. ; Suzuki, Shigeki ; Yao, Xiaomei ; Wang, Zhigang ; Wang, Yong ; Honeycutt, Cherlita ; Iozzo, Renato V. ; Young, Marian F. ; Kulkarni, Ashok B. / Genetic evidence for key roles of decorin and biglycan in dentin mineralization. In: Matrix Biology. 2009 ; Vol. 28, No. 3. pp. 129-136.
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