Genetic evidence for Shc requirement in TCR-induced c-Rel nuclear translocation and IL-2 expression

Makio Iwashima, Masako Takamatsu, Hiroko Yamagishi, Yasue Hatanaka, Yi Ying Huang, Courtnie McGinty, Sho Yamasaki, Toru Koike

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    24 Citations (Scopus)

    Abstract

    Shc, a prototypic adapter molecule, has been implicated in T cell receptor (TCR) signal transduction, but its role has not been identified clearly. Here we report that Shc is essential for TCR-induced IL-2 production but is dispensable for CD69 or CD25 expression. Engagement of TCR in mutant Jurkat T cells lacking Shc fails to produce IL-2 because of impaired mitogen-activated protein kinase activation. Activation of c-Rel, a transcription factor essential for IL-2 expression, was impaired also. In contrast, activation of nuclear factor of activated T cell and expression of CD69/CD25 were comparable between the mutant and wild-type Jurkat cells. These defects were rescued by expression of exogenous Shc. Activation of c-Rel using the estrogen receptor fusion protein restored the activation of the IL-2 promoter in an estrogen-dependent manner. These results show that Shc plays an essential role in the TCR-induced activation of c-Rel and the IL-2 promoter.

    Original languageEnglish
    Pages (from-to)4544-4549
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume99
    Issue number7
    DOIs
    Publication statusPublished - Apr 2 2002

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