TY - JOUR
T1 - Genetic evidence linking SAP, the X-linked lymphoproliferative gene product, to Src-related kinase FynT in TH2 cytokine regulation
AU - Davidson, Dominique
AU - Shi, Xiaochu
AU - Zhang, Shaohua
AU - Wang, Hao
AU - Nemer, Mona
AU - Ono, Nobuyuki
AU - Ohno, Shinji
AU - Yanagi, Yusuke
AU - Veillette, André
N1 - Funding Information:
We thank Sylvain Latour for useful discussions. We also thank Pamela Schwartzberg for sap − mouse and useful discussions and Eric Massicotte and Martine Dupuis for cell sorting. This work was supported by grants from the Canadian Institutes of Health Research (to A.V. and M.N.), the National Cancer Institute of Canada (to A.V.), and the CANVAC National Centre of Excellence (to A.V.). H.W. holds a Fellowship of the Heart and Stroke Foundation of Canada. A.V. and M.N. are Senior Investigators of the Canadian Institutes of Health Research. M.N. holds the Canada Research Chair in Cardiac Differentiation and Growth Mechanism, while A.V. holds the Canada Research Chair in Signaling in the Immune System.
PY - 2004/11
Y1 - 2004/11
N2 - SAP is an adaptor mutated in X-linked lymphoproliferative disease. It plays a critical role in T helper 2 (TH2) cytokine production. This function was suggested to reflect the capacity of SAP to associate with SLAM family receptors and enable tyrosine phosphorylation signaling by these receptors through SAP-mediated recruitment of Src-related kinase FynT. Here, we addressed by genetic means the importance of the SAP-FynT interaction in normal T cell functions. By creating a mouse in which the FynT binding site of SAP was inactivated in the germ line (sapR78A mouse) and by analyzing mice lacking SAP, FynT or SLAM, evidence was obtained that the SAP-FynT cascade is indeed crucial for normal TH2 functions in vitro and in vivo. These data imply that SAP is necessary for TH2 cytokine regulation primarily as a result of its capacity to recruit FynT. They also establish a previously unappreciated role for FynT in SAP-dependent TH2 cytokine regulation.
AB - SAP is an adaptor mutated in X-linked lymphoproliferative disease. It plays a critical role in T helper 2 (TH2) cytokine production. This function was suggested to reflect the capacity of SAP to associate with SLAM family receptors and enable tyrosine phosphorylation signaling by these receptors through SAP-mediated recruitment of Src-related kinase FynT. Here, we addressed by genetic means the importance of the SAP-FynT interaction in normal T cell functions. By creating a mouse in which the FynT binding site of SAP was inactivated in the germ line (sapR78A mouse) and by analyzing mice lacking SAP, FynT or SLAM, evidence was obtained that the SAP-FynT cascade is indeed crucial for normal TH2 functions in vitro and in vivo. These data imply that SAP is necessary for TH2 cytokine regulation primarily as a result of its capacity to recruit FynT. They also establish a previously unappreciated role for FynT in SAP-dependent TH2 cytokine regulation.
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U2 - 10.1016/j.immuni.2004.10.005
DO - 10.1016/j.immuni.2004.10.005
M3 - Article
C2 - 15539156
AN - SCOPUS:8444231176
VL - 21
SP - 707
EP - 717
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 5
ER -
