Genetic variation in food choice behaviour of amino acid-deprived Drosophila

Naoko Toshima, Chieko Hara, Claus Jürgen Scholz, Teiichi Tanimura

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

To understand homeostatic regulation in insects, we need to understand the mechanisms by which they respond to external stimuli to maintain the internal milieu. Our previous study showed that Drosophila melanogaster exhibit specific amino acid preferences. Here, we used the D. melanogaster Genetic Reference Panel (DGRP), which is comprised of multiple inbred lines derived from a natural population, to examine how amino acid preference changes depending on the internal nutritional state in different lines. We performed a two-choice preference test and observed genetic variations in the response to amino acid deprivation. For example, a high-responding line showed an enhanced preference for amino acids even after only 1. day of deprivation and responded to a fairly low concentration of amino acids. Conversely, a low-responding line showed no increased preference for amino acids after deprivation. We compared the gene expression profiles between selected high- and the low-responding lines and performed SNP analyses. We found several groups of genes putatively involved in altering amino acid preference. These results will contribute to future studies designed to explore how the genetic architecture of an organism evolves to adapt to different nutritional environments.

Original languageEnglish
Pages (from-to)89-94
Number of pages6
JournalJournal of insect physiology
Volume69
Issue numberC
DOIs
Publication statusPublished - Jan 1 2014

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Choice Behavior
food choices
Drosophila
Amino Acids
Food
genetic variation
amino acids
Drosophila melanogaster
Transcriptome
inbred lines
Single Nucleotide Polymorphism
Insects
nutritional status
gene expression
insects
organisms

All Science Journal Classification (ASJC) codes

  • Physiology
  • Insect Science

Cite this

Genetic variation in food choice behaviour of amino acid-deprived Drosophila. / Toshima, Naoko; Hara, Chieko; Scholz, Claus Jürgen; Tanimura, Teiichi.

In: Journal of insect physiology, Vol. 69, No. C, 01.01.2014, p. 89-94.

Research output: Contribution to journalArticle

Toshima, Naoko ; Hara, Chieko ; Scholz, Claus Jürgen ; Tanimura, Teiichi. / Genetic variation in food choice behaviour of amino acid-deprived Drosophila. In: Journal of insect physiology. 2014 ; Vol. 69, No. C. pp. 89-94.
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