Genetic variation in populations of Allothrombium pulvinum (Acari: Trombidiidae) from Northern Iran revealed by mitochondrial coxI and nuclear rDNA ITS2 sequences

Marjan Khalili Mahani, Nobuyuki Inomata, Alireza Saboori, Baraldin Ebrahim Sayed Tabatabaei, Hiroko Ishiyama, Ardeshir Ariana, Alfred Szmidt

Research output: Contribution to journalArticle

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Abstract

Allothrombium pulvinum Ewing is a common natural enemy of aphids and some other arthropods. So far, there are no studies that have addressed genetic variation of this predatory mite. We investigated genetic variation of A. pulvinum across its whole known range in Iran. A 410 bp portion of the mitochondrial cytochrome c oxidase subunit I gene (coxI) and 797-802 bp portion of the internal transcribed spacer 2 of rDNA (ITS2) were sequenced for 55 individuals from 11 populations, resulting in 12 and 26 haplotypes, respectively. In the coxI region, haplotype and nucleotide diversities varied among populations from 0.00 to 0.90 and from 0.0000 to 0.0110, respectively. In the ITS2 region they varied from 0.20 to 0.91 and from 0.0006 to 0.0023, respectively. For both gene regions the highest haplotype and nucleotide diversities were detected in population Mahmoud Abad from northern Iran. Statistically significant population differentiation (F ST) was detected in most pair-wise population comparisons. The results of population differentiation for both gene regions were generally congruent indicating that A. pulvinum from Iran consists of genetically different populations. This suggests that A. pulvinum comprises at least two geographically distinct populations or even more than one species. This study is an initial step towards understanding genetic variation of A. pulvinum, a taxon for which little molecular information is available. More intensive sampling and analysis of additional DNA regions are necessary for more detailed classification of this taxon.

Original languageEnglish
Pages (from-to)273-289
Number of pages17
JournalExperimental and Applied Acarology
Volume48
Issue number4
DOIs
Publication statusPublished - Aug 1 2009

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Allothrombium
Trombidiidae
internal transcribed spacers
genetic variation
Acari
Iran
gene
haplotypes
natural enemy
aphid
mite
nucleotides
arthropod
cytochrome
genes
predatory mites
DNA
natural enemies
cytochrome-c oxidase
arthropods

All Science Journal Classification (ASJC) codes

  • Ecology
  • Insect Science

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Genetic variation in populations of Allothrombium pulvinum (Acari : Trombidiidae) from Northern Iran revealed by mitochondrial coxI and nuclear rDNA ITS2 sequences. / Khalili Mahani, Marjan; Inomata, Nobuyuki; Saboori, Alireza; Sayed Tabatabaei, Baraldin Ebrahim; Ishiyama, Hiroko; Ariana, Ardeshir; Szmidt, Alfred.

In: Experimental and Applied Acarology, Vol. 48, No. 4, 01.08.2009, p. 273-289.

Research output: Contribution to journalArticle

Khalili Mahani, Marjan ; Inomata, Nobuyuki ; Saboori, Alireza ; Sayed Tabatabaei, Baraldin Ebrahim ; Ishiyama, Hiroko ; Ariana, Ardeshir ; Szmidt, Alfred. / Genetic variation in populations of Allothrombium pulvinum (Acari : Trombidiidae) from Northern Iran revealed by mitochondrial coxI and nuclear rDNA ITS2 sequences. In: Experimental and Applied Acarology. 2009 ; Vol. 48, No. 4. pp. 273-289.
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