Genetic variation of glutelin acidic subunit polypeptides in Bangladesh rice genetic resources

Md Sarwar Jahan, Yuji Uemura, Toshihiro Kumamaru, Abdul Hamid, Hikaru Satoh

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Bangladesh rice genetic resources collected from six distinct regions of the country were examined to obtain the diversity in glutelin acidic subunit polypeptides. Seed glutelins from 576 Bangladesh rice cultivars representing seven ecotypes were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focusing (IEF) electrophoresis and two-dimensional electrophoresis (2-DE: SDS-PAGE/IEF) analyses. Glutelin acidic subunit was separated into four bands, α-1, α-2, α-3 and α-4, and the variation in each of bands was detected by SDS-PAGE analysis. A higher molecular size component of α-1(α-1′) was identified in the cultivars tested. In case of α-2, α-2H with high molecular mass, α-2L with low molecular mass and α-2H/L were detected. α-3 band variation showed α-3H, α-3L, and α-3H/L, while for α-4, α-4H, α-4L bands were identified. In IEF analysis, a total of 16 bands with independent pI ranging from pI 6.30 to 7.52 were identified for the glutelin acidic subunit among the cultivars. The maximum and minimum numbers of IEF bands found were 13 and 9, respectively. The α-2L less cultivars were also lacking in pI 6.80 polypeptide in IEF. Result of 2-DE showed that pI 6.80 polypeptide was the main component of α-2L band. Transplanted Aman ecotype was the most diverse with respect to glutelin variation. Geographical distribution of glutelin variation in the Transplanted Aman ecotype differed according to the regions. The above results indicate that Bangladesh rice cultivars possess great genetic diversity in glutelin acidic subunit polypeptides. This study of indigenous rice cultivars from Bangladesh provides useful information regarding their breeding potential.

Original languageEnglish
Pages (from-to)977-987
Number of pages11
JournalGenetic Resources and Crop Evolution
Volume52
Issue number8
DOIs
Publication statusPublished - Dec 1 2005

Fingerprint

glutelins
Bangladesh
Glutens
genetic resource
genetic resources
genetic variation
cultivar
polypeptides
isoelectric focusing
rice
Isoelectric Focusing
electrokinesis
Peptides
Ecotype
ecotype
cultivars
ecotypes
Sodium Dodecyl Sulfate
gel
polyacrylamide gel electrophoresis

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Genetic variation of glutelin acidic subunit polypeptides in Bangladesh rice genetic resources. / Jahan, Md Sarwar; Uemura, Yuji; Kumamaru, Toshihiro; Hamid, Abdul; Satoh, Hikaru.

In: Genetic Resources and Crop Evolution, Vol. 52, No. 8, 01.12.2005, p. 977-987.

Research output: Contribution to journalArticle

Jahan, Md Sarwar ; Uemura, Yuji ; Kumamaru, Toshihiro ; Hamid, Abdul ; Satoh, Hikaru. / Genetic variation of glutelin acidic subunit polypeptides in Bangladesh rice genetic resources. In: Genetic Resources and Crop Evolution. 2005 ; Vol. 52, No. 8. pp. 977-987.
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