Genetic variations in the vitellogenin of Japanese populations of the wild silkworm Bombyx mandarina

Yan Meng, Chaoliang Liu, Kunihiro Shiomi, Masao Nakagaki, Yutaka Banno, Zenta Kajiura

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We cloned and sequenced cDNA of Bombyx mandarina vitellogenin (BmaVg) inhabiting Japan. The complete sequence of BmaVg cDNA without a poly A tall is 5733 bp long and encodes 1780 amino acid residues among which are typical highly conserved sequences and common features found in most insects. Multiple alignment of vitellogenin sequences of Japanese Bombyx mandarina, the Chinese one, and Bombyx mori showed that the nucleotide sequence of Japanese BmaVg cDNA shared 99.5% identity with Chinese BmaVg and 98.2% identity with Bombyx mori vitellogenin (BmoVg), and that the amino acid sequence of Japanese BmaVg cDNA shared 99.6% homology with Chinese BmaVg and 97.5% homology with BmoVg. These findings indicate that BmaVg (JPN) is more similar to BmaVg (CHN) than to BmoVg, which was also confirmed through a comparison of the mutation frequencies of both nucleotide and amino acid sequences. The phylogenetic tree of Vgs demonstrates that BmaVgs of individuals collected from eighteen prefectures in Japan primarily cluster in a group with BmaVg (CHN) and without BmoVg, suggesting that the sequence variation between the two BmaVgs arose after divergence of B. mori from B. mandarina.

Original languageEnglish
Pages (from-to)127-134
Number of pages8
JournalJournal of Insect Biotechnology and Sericology
Volume75
Issue number3
Publication statusPublished - Oct 1 2006

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Vitellogenins
Bombyx
vitellogenin
Amino acids
Nucleotides
genetic variation
Population
Bombyx mori
Complementary DNA
Bombyx mandarina
Silkworm
amino acid sequences
Amino Acid Sequence
Japan
cDNA
conserved sequences
Poly A
Conserved Sequence
Sequence Alignment
Mutation Rate

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Insect Science
  • Industrial and Manufacturing Engineering

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Genetic variations in the vitellogenin of Japanese populations of the wild silkworm Bombyx mandarina. / Meng, Yan; Liu, Chaoliang; Shiomi, Kunihiro; Nakagaki, Masao; Banno, Yutaka; Kajiura, Zenta.

In: Journal of Insect Biotechnology and Sericology, Vol. 75, No. 3, 01.10.2006, p. 127-134.

Research output: Contribution to journalArticle

Meng, Yan ; Liu, Chaoliang ; Shiomi, Kunihiro ; Nakagaki, Masao ; Banno, Yutaka ; Kajiura, Zenta. / Genetic variations in the vitellogenin of Japanese populations of the wild silkworm Bombyx mandarina. In: Journal of Insect Biotechnology and Sericology. 2006 ; Vol. 75, No. 3. pp. 127-134.
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