Genetically engineered nanofiber-like viruses for tissue regenerating materials

Anna Merzlyak, Shyam Indrakanti, Seung Wuk Lee

Research output: Contribution to journalArticlepeer-review

162 Citations (Scopus)

Abstract

Controlling structural organization and signaling motif display of biomimetic matrices at the nanometer scale Is of great Importance to the functional design of tissue regenerating materials. We have genetically engineered M13 bacteriophage (phage), naturally occurring nanoflber-like viruses, to display a high density of cell-signaling peptides on their major coat proteins. Structural orientation of these phage building blocks can be achieved due to their long-rod shape and monodlsperslty, which lead them to self-assemble Into dlrectlonally organized liquid crystalline-like materials. We showed that the constructed viral nanofiber scaffolds were able to support neural progenitor cell proliferation and differentiation as well as direct orientation of their growth in three dimensions. Such functionalized and structurally aligned phage matrices offer promising opportunities for therapies that address challenging medical problems, such as nerve tissue regeneration after spinal cord injuries, or as in vitro model systems for studying complicated cell signaling environments.

Original languageEnglish
Pages (from-to)846-852
Number of pages7
JournalNano Letters
Volume9
Issue number2
DOIs
Publication statusPublished - Feb 11 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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