Genetically fused charged peptides induce rapid crystallization of proteins

K. Minamihata; K. Tsukamoto; M. Adachi; R. Shimizu; M. Mishina; R. Kuroki; T. Nagamune

doi:10.1039/c9cc09529b

Genetically fused charged peptides induce rapid crystallization of proteins

K. Minamihata, K. Tsukamoto, M. Adachi, R. Shimizu, M. Mishina, R. Kuroki, T. Nagamune

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We utilized electrostatic interaction to induce rapid crystallization of streptavidin. Simply mixing streptavidins possessing either a positively or negatively charged peptide at their C-terminus generated diffraction-quality crystals in a few hours. We modified the streptavidin crystals with fluorescent molecules using biotin, demonstrating the concept of protein crystals as functional biomaterials.

Original language	English
Pages (from-to)	3891-3894
Number of pages	4
Journal	Chemical Communications
Volume	56
Issue number	27
DOIs	https://doi.org/10.1039/c9cc09529b
Publication status	Published - Apr 7 2020

All Science Journal Classification (ASJC) codes

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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AU - Minamihata, K.

AU - Tsukamoto, K.

AU - Adachi, M.

AU - Shimizu, R.

AU - Mishina, M.

AU - Kuroki, R.

AU - Nagamune, T.

N1 - Publisher Copyright: © The Royal Society of Chemistry.

PY - 2020/4/7

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AB - We utilized electrostatic interaction to induce rapid crystallization of streptavidin. Simply mixing streptavidins possessing either a positively or negatively charged peptide at their C-terminus generated diffraction-quality crystals in a few hours. We modified the streptavidin crystals with fluorescent molecules using biotin, demonstrating the concept of protein crystals as functional biomaterials.

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Genetically fused charged peptides induce rapid crystallization of proteins

Abstract

All Science Journal Classification (ASJC) codes

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