TY - JOUR
T1 - Genipin-stabilized caseinatehitosan nanoparticles for enhanced stability and anti-cancer activity of curcumin
AU - Razi, Muhamad Alif
AU - Wakabayashi, Rie
AU - Tahara, Yoshiro
AU - Goto, Masahiro
AU - Kamiya, Noriho
N1 - Funding Information:
This work was supported by JSPS KAKENHI Grant Numbers JP16H06369 and JP16H04581 . A scholarship from the Ministry of Education, Cultures, Sports, Science and Technology (MEXT) provided to M.A.R (Scholarship ID No. 150043) is deeply appreciated. We thank Edanz Group ( www.edanzediting.com ) for editing a draft of this manuscript. We appreciated the technical assistance for flow cytometric analysis from The Research Support Center, Kyushu University Graduate School of Medical Sciences.
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/4/1
Y1 - 2018/4/1
N2 - Nanoparticles formed by the assembly of protein and polysaccharides are of great interest for the delivery of hydrophobic molecules. Herein, the formation of genipin-crosslinked nanoparticles from caseinate (CS) and chitosan (CH) is reported for the delivery of curcumin, a polyphenolic compound from turmeric, to cells. Genipin-crosslinked CS-CH nanoparticles (G-CCNPs) having a diameter of ∼250 nm and a low polydispersity index showed excellent stability over a wide pH range, as indicated by dynamic light scattering and transmission electron microscopic measurements. Cellular uptake of curcumin loaded into G-CCNPs by HeLa cells was improved, as measured by confocal laser scanning microscopy (CLSM) and fluorescence-activated cell-sorting analysis. Cell proliferation assays indicated that G-CCNPs were nontoxic and that curcumin's anticancer activity in vitro was also improved by G-CCNPs. Stability of curcumin at neutral pH was enhanced by G-CCNPs. CLSM study revealed that G-CCNPs were poorly internalized by HeLa cells, possibly because of strong cell membrane interactions and a negative zeta potential. Overall, our results suggested that the enhanced curcumin cytotoxicity might be associated with the enhanced stability of curcumin by G-CCNPs and free curcumin released from G-CCNPs into the cell. These biocompatible NPs might be suitable carriers for enhancing curcumin's therapeutic potential.
AB - Nanoparticles formed by the assembly of protein and polysaccharides are of great interest for the delivery of hydrophobic molecules. Herein, the formation of genipin-crosslinked nanoparticles from caseinate (CS) and chitosan (CH) is reported for the delivery of curcumin, a polyphenolic compound from turmeric, to cells. Genipin-crosslinked CS-CH nanoparticles (G-CCNPs) having a diameter of ∼250 nm and a low polydispersity index showed excellent stability over a wide pH range, as indicated by dynamic light scattering and transmission electron microscopic measurements. Cellular uptake of curcumin loaded into G-CCNPs by HeLa cells was improved, as measured by confocal laser scanning microscopy (CLSM) and fluorescence-activated cell-sorting analysis. Cell proliferation assays indicated that G-CCNPs were nontoxic and that curcumin's anticancer activity in vitro was also improved by G-CCNPs. Stability of curcumin at neutral pH was enhanced by G-CCNPs. CLSM study revealed that G-CCNPs were poorly internalized by HeLa cells, possibly because of strong cell membrane interactions and a negative zeta potential. Overall, our results suggested that the enhanced curcumin cytotoxicity might be associated with the enhanced stability of curcumin by G-CCNPs and free curcumin released from G-CCNPs into the cell. These biocompatible NPs might be suitable carriers for enhancing curcumin's therapeutic potential.
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U2 - 10.1016/j.colsurfb.2018.01.041
DO - 10.1016/j.colsurfb.2018.01.041
M3 - Article
C2 - 29413610
AN - SCOPUS:85041473111
VL - 164
SP - 308
EP - 315
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -
