Genome editing of pufferfish saxitoxin- and tetrodotoxin-binding protein type 2 in Takifugu rubripes

Yoko Kato-Unoki, Yuki Takai, Masato Kinoshita, Toshitaka Mochizuki, Ryohei Tatsuno, Yohei Shimasaki, Yuji Oshima

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The pufferfish saxitoxin- and tetrodotoxin-binding protein 2 (PSTBP2), which is involved in toxin accumulation, was knocked out in Takifugu rubripes embryos by using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 genome-editing technology. Treating the embryos with one of two single-guide RNA (sgRNA) resulted in mutation rates of 57.1% and 62.5%, respectively, as estimated using a heteroduplex mobility assay at 3 days postfertilization. Both sgRNAs might induced frameshift mutations that knocked out the T. rubripes PSTBP2.

Original languageEnglish
Pages (from-to)58-61
Number of pages4
JournalToxicon
Volume153
DOIs
Publication statusPublished - Oct 2018

All Science Journal Classification (ASJC) codes

  • Toxicology

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