Genome sequencing verifies relapsed infection of helicobacter cinaedi

Osamu Sawada, Yasuhiro Gotoh, Takako Taniguchi, Shota Furukawa, Dai Yoshimura, Satomi Sasaki, Haruki Shida, Yoshihiro Kusunoki, Tsuyoshi Yamamura, Ken Furuya, Takehiko Itoh, Tetsuya Horita, Tetsuya Hayashi, Naoaki Misawa

Research output: Contribution to journalArticle

Abstract

Background. Recurrent infections of Helicobacter cinaedi are often reported, and long-term antimicrobial treatment is empirically recommended to prevent such infections. However, there have been no studies examining whether recurrent infections are relapses of former infections or reinfections with different clones. Methods. A 69-year-old woman presented with recurrent H cinaedi bacteremia-associated cellulitis after a 51-day interval. We isolated 10 colonies from the blood cultures obtained during each of the 2 episodes and subjected them to whole-genome sequencing (WGS). High-confidence single-nucleotide polymorphisms (SNPs) were identified by an assembly based method. Heterogeneous SNP sites were identified by read mapping. The susceptibility of a representative isolate to 14 antimicrobials was also examined. Results. Whole-genome sequence analysis revealed only 6 SNP sites among the 20 isolates at the whole-genome level. Based on the 6 SNPs, 5 within-host variants (referred to as genotypes) were identified. All 5 genotypes were detected in the first infection; however, only 2 genotypes were detected in the second infection. Although the H cinaedi clone showed a higher minimum inhibitory concentration to fluoroquinolones and macrolides and responsible mutations were identified, none of the 6 SNPs appeared related to additional resistance. Conclusions. The second infection analyzed here was a relapse of the first infection. A certain level of within-host genomic heterogeneity of the H cinaedi clone was already present in the first infection. Our results suggest the importance of longer treatment courses to eradicate H cinaedi for preventing the relapse of its infection.

Original languageEnglish
JournalOpen Forum Infectious Diseases
Volume6
Issue number5
DOIs
Publication statusPublished - Jan 1 2019

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Helicobacter Infections
Genome
Infection
Single Nucleotide Polymorphism
Clone Cells
Genotype
Recurrence
Cellulitis
Fluoroquinolones
Macrolides
Microbial Sensitivity Tests
Bacteremia
Sequence Analysis
Mutation
Therapeutics

All Science Journal Classification (ASJC) codes

  • Oncology
  • Clinical Neurology

Cite this

Sawada, O., Gotoh, Y., Taniguchi, T., Furukawa, S., Yoshimura, D., Sasaki, S., ... Misawa, N. (2019). Genome sequencing verifies relapsed infection of helicobacter cinaedi. Open Forum Infectious Diseases, 6(5). https://doi.org/10.1093/ofid/ofz200

Genome sequencing verifies relapsed infection of helicobacter cinaedi. / Sawada, Osamu; Gotoh, Yasuhiro; Taniguchi, Takako; Furukawa, Shota; Yoshimura, Dai; Sasaki, Satomi; Shida, Haruki; Kusunoki, Yoshihiro; Yamamura, Tsuyoshi; Furuya, Ken; Itoh, Takehiko; Horita, Tetsuya; Hayashi, Tetsuya; Misawa, Naoaki.

In: Open Forum Infectious Diseases, Vol. 6, No. 5, 01.01.2019.

Research output: Contribution to journalArticle

Sawada, O, Gotoh, Y, Taniguchi, T, Furukawa, S, Yoshimura, D, Sasaki, S, Shida, H, Kusunoki, Y, Yamamura, T, Furuya, K, Itoh, T, Horita, T, Hayashi, T & Misawa, N 2019, 'Genome sequencing verifies relapsed infection of helicobacter cinaedi', Open Forum Infectious Diseases, vol. 6, no. 5. https://doi.org/10.1093/ofid/ofz200
Sawada, Osamu ; Gotoh, Yasuhiro ; Taniguchi, Takako ; Furukawa, Shota ; Yoshimura, Dai ; Sasaki, Satomi ; Shida, Haruki ; Kusunoki, Yoshihiro ; Yamamura, Tsuyoshi ; Furuya, Ken ; Itoh, Takehiko ; Horita, Tetsuya ; Hayashi, Tetsuya ; Misawa, Naoaki. / Genome sequencing verifies relapsed infection of helicobacter cinaedi. In: Open Forum Infectious Diseases. 2019 ; Vol. 6, No. 5.
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AU - Taniguchi, Takako

AU - Furukawa, Shota

AU - Yoshimura, Dai

AU - Sasaki, Satomi

AU - Shida, Haruki

AU - Kusunoki, Yoshihiro

AU - Yamamura, Tsuyoshi

AU - Furuya, Ken

AU - Itoh, Takehiko

AU - Horita, Tetsuya

AU - Hayashi, Tetsuya

AU - Misawa, Naoaki

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