TY - JOUR
T1 - Genome-wide analysis of chromatin structure changes upon MyoD binding in proliferative myoblasts during the cell cycle
AU - Wu, Qianmei
AU - Fujii, Takeru
AU - Harada, Akihito
AU - Tomimatsu, Kosuke
AU - Miyawaki-Kuwakado, Atsuko
AU - Fujita, Masatoshi
AU - Maehara, Kazumitsu
AU - Ohkawa, Yasuyuki
N1 - Funding Information:
This work was in part supported by the Japan Science and Technology Agency PRESTO JPMJPR2026 to K.M., JPMJPR19K7 to A.H., Japan Science and Technology Agency CREST JPMJCR16G1 to Y.O.; The Ministry of Education, Culture, Sports, Science and Technology/Japan Society for the Promotion of Science KAKENHI JP19H04970, JP19H03158 and JP20H05393 to K.M.; JP18K19432, JP19H03211, JP19H05425 and JP20H05368 to A.H.; JP18H04802, JP18H05527, JP19H05244, JP17H03608, JP20H00456 and JP20H04846 to Y.O.; Japan Agency for Medical Research and Development JP20ek0109489h0001 to Y.O.
Publisher Copyright:
© 2021 The Author(s) 2021.
PY - 2021/6/1
Y1 - 2021/6/1
N2 - MyoD, a myogenic differentiation protein, has been studied for its critical role in skeletal muscle differentiation. MyoD-expressing myoblasts have a potency to be differentiated with proliferation of ectopic cells. However, little is known about the effect on chromatin structure of MyoD binding in proliferative myoblasts. In this study, we evaluated the chromatin structure around MyoD-bound genome regions during the cell cycle by chromatin immunoprecipitation sequencing. Genome-wide analysis of histone modifications was performed in proliferative mouse C2C12 myoblasts during three phases (G1, S, G2/M) of the cell cycle. We found that MyoD-bound genome regions had elevated levels of active histone modifications, such as H3K4me1/2/3 and H3K27ac, compared with MyoD-unbound genome regions during the cell cycle. We also demonstrated that the elevated H3K4me2/3 modification level was maintained during the cell cycle, whereas the H3K27ac and H3K4me1 modification levels decreased to the same level as MyoD-unbound genome regions during the later phases. Immunoblot analysis revealed that MyoD abundance was high in the G1 phase then decreased in the S and G2/M phases. Our results suggest that MyoD binding formed selective epigenetic memories with H3K4me2/3 during the cell cycle in addition to myogenic gene induction via active chromatin formation coupled with transcription.
AB - MyoD, a myogenic differentiation protein, has been studied for its critical role in skeletal muscle differentiation. MyoD-expressing myoblasts have a potency to be differentiated with proliferation of ectopic cells. However, little is known about the effect on chromatin structure of MyoD binding in proliferative myoblasts. In this study, we evaluated the chromatin structure around MyoD-bound genome regions during the cell cycle by chromatin immunoprecipitation sequencing. Genome-wide analysis of histone modifications was performed in proliferative mouse C2C12 myoblasts during three phases (G1, S, G2/M) of the cell cycle. We found that MyoD-bound genome regions had elevated levels of active histone modifications, such as H3K4me1/2/3 and H3K27ac, compared with MyoD-unbound genome regions during the cell cycle. We also demonstrated that the elevated H3K4me2/3 modification level was maintained during the cell cycle, whereas the H3K27ac and H3K4me1 modification levels decreased to the same level as MyoD-unbound genome regions during the later phases. Immunoblot analysis revealed that MyoD abundance was high in the G1 phase then decreased in the S and G2/M phases. Our results suggest that MyoD binding formed selective epigenetic memories with H3K4me2/3 during the cell cycle in addition to myogenic gene induction via active chromatin formation coupled with transcription.
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U2 - 10.1093/jb/mvab001
DO - 10.1093/jb/mvab001
M3 - Article
C2 - 33479729
AN - SCOPUS:85116326959
VL - 169
SP - 653
EP - 661
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 6
ER -