Genome-wide analysis of histone modifications in human endometrial stromal cells

Isao Tamura, Yasuyuki Ohkawa, Tetsuya Sato, Mikita Suyama, Kosuke Jozaki, Maki Okada, Lifa Lee, Ryo Maekawa, Hiromi Asada, Shun Sato, Yoshiaki Yamagata, Hiroshi Tamura, Norihiro Sugino

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Dramatic changes of gene expressions occur in human endometrial stromal cells (ESCs) during decidualization. The changes in gene expression are associated with changes of chromatin structure, which are regulated by histone modifications. Here we investigated genome-wide changes in histone modifications associated with decidualization in human ESCs using chromatin immunoprecipitation combined with next-generation sequencing. ESCs were incubated with estradiol and medroxyprogesterone acetate for 14 days to induce decidualization. The chromatin immunoprecipitation- sequence data showed that induction of decidualization increased H3K27ac and H3K4me3 signals in many genomic regions but decreased in only a few regions. Most of the H3K27ac-increased regions (80%) and half of the H3K4me3-increased regions were located in the distal promoter regions (more than 3 kb upstream or downstream of the transcription start site). RNA sequence showed that induction of decidualization up-regulated 881 genes, 223 of which had H3K27ac- or H3K4me3-increased regions in the proximal and distal promoter regions. Induction of decidualization increased themRNAlevels of these genes more than it increased themRNA levels of genes without H3K27ac- or H3K4me3-increased regions. Pathway analysis revealed that up-regulated genes with the H3K27ac- or H3K4me3-increased regions were associated with the insulin signaling, which may be involved in glucose uptake that is necessary for ESCs to undergo decidualization. These results show that histone modification statuses on a genome-wide basis change in human ESCs during decidualization. The main changes of histone modifications are increases of H3K27ac and H3K4me3 in both the proximal and distal promoter regions, which are involved in the up-regulation of gene expression that occurs during decidualization.

Original languageEnglish
Pages (from-to)1656-1669
Number of pages14
JournalMolecular Endocrinology
Volume28
Issue number10
DOIs
Publication statusPublished - Oct 1 2014

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Histone Code
Stromal Cells
Genome
Genetic Promoter Regions
Chromatin Immunoprecipitation
Genes
Gene Expression
Medroxyprogesterone Acetate
Transcription Initiation Site
Gene Expression Regulation
Chromatin
Estradiol
Up-Regulation
Insulin
Glucose

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Endocrinology

Cite this

Genome-wide analysis of histone modifications in human endometrial stromal cells. / Tamura, Isao; Ohkawa, Yasuyuki; Sato, Tetsuya; Suyama, Mikita; Jozaki, Kosuke; Okada, Maki; Lee, Lifa; Maekawa, Ryo; Asada, Hiromi; Sato, Shun; Yamagata, Yoshiaki; Tamura, Hiroshi; Sugino, Norihiro.

In: Molecular Endocrinology, Vol. 28, No. 10, 01.10.2014, p. 1656-1669.

Research output: Contribution to journalArticle

Tamura, I, Ohkawa, Y, Sato, T, Suyama, M, Jozaki, K, Okada, M, Lee, L, Maekawa, R, Asada, H, Sato, S, Yamagata, Y, Tamura, H & Sugino, N 2014, 'Genome-wide analysis of histone modifications in human endometrial stromal cells', Molecular Endocrinology, vol. 28, no. 10, pp. 1656-1669. https://doi.org/10.1210/me.2014-1117
Tamura, Isao ; Ohkawa, Yasuyuki ; Sato, Tetsuya ; Suyama, Mikita ; Jozaki, Kosuke ; Okada, Maki ; Lee, Lifa ; Maekawa, Ryo ; Asada, Hiromi ; Sato, Shun ; Yamagata, Yoshiaki ; Tamura, Hiroshi ; Sugino, Norihiro. / Genome-wide analysis of histone modifications in human endometrial stromal cells. In: Molecular Endocrinology. 2014 ; Vol. 28, No. 10. pp. 1656-1669.
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