Genome-wide identification of Argonaute 1- and Argonaute 2-regulating genes revealed an inhibition of macula-like virus by RNAi pathway in the silkworm, Bombyx mori

Li Zhu, Zhiqing Li, Tsuneyuki Tatsuke, Daojun Cheng, Jian Xu, Kaito Yoshimura, Hiroaki Mon, Kazuhiro Iiyama, Man Lee, Qingyou Xia, Takahiro Kusakabe

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Using a cDNA microarray, we monitored global gene expression profiles in silkworm BmN4-SID1 cells after the knockdown of BmAGO1 and BmAGO2 genes. Interestingly, there was a significant overlap between the target genes up-regulated by BmAgo1 (292 out of 481) and BmAgo2 (292 out of 361). Of these, the Replicase gene of macula-like virus (BmMLV) was highly induced in both of BmAgo1- and BmAgo2-depleted cells. RT-PCR analysis confirmed that the knockdown of silkworm RNAi pathway-related genes increased the expression of the Replicase gene. These data strongly suggested that the RNAi pathway negatively regulated BmMLV proliferation in silkworm BmN4 cells.

Original languageEnglish
Pages (from-to)19-23
Number of pages5
JournalJournal of Insect Biotechnology and Sericology
Volume82
Issue number1
DOIs
Publication statusPublished - Aug 5 2013

Fingerprint

Bombyx
Bombyx mori
RNA Interference
silkworms
Viruses
Genes
Genome
viruses
genome
genes
gene expression
cells
Oligonucleotide Array Sequence Analysis
Transcriptome
Microarrays
Gene expression
reverse transcriptase polymerase chain reaction
Gene Expression
Polymerase Chain Reaction
Silkworm

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Insect Science
  • Industrial and Manufacturing Engineering

Cite this

Genome-wide identification of Argonaute 1- and Argonaute 2-regulating genes revealed an inhibition of macula-like virus by RNAi pathway in the silkworm, Bombyx mori. / Zhu, Li; Li, Zhiqing; Tatsuke, Tsuneyuki; Cheng, Daojun; Xu, Jian; Yoshimura, Kaito; Mon, Hiroaki; Iiyama, Kazuhiro; Lee, Man; Xia, Qingyou; Kusakabe, Takahiro.

In: Journal of Insect Biotechnology and Sericology, Vol. 82, No. 1, 05.08.2013, p. 19-23.

Research output: Contribution to journalArticle

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AU - Cheng, Daojun

AU - Xu, Jian

AU - Yoshimura, Kaito

AU - Mon, Hiroaki

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