Genome-wide transcriptional orchestration of circadian rhythms in Drosophila

Hiroki R. Ueda, Akira Matsumoto, Miho Kawamura, Masamitsu Iino, Teiichi Tanimura, Seiichi Hashimoto

Research output: Contribution to journalArticle

226 Citations (Scopus)

Abstract

Circadian rhythms govern the behavior, physiology, and metabolism of living organisms. Recent studies have revealed the role of several genes in the clock mechanism both in Drosophila and in mammals. To study how gene expression is globally regulated by the clock mechanism, we used a high density oligonucleotide probe array (GeneChip) to profile gene expression patterns in Drosophila under light-dark and constant dark conditions. We found 712 genes showing a daily fluctuation in mRNA levels under light-dark conditions, and among these the expression of 115 genes was still cycling in constant darkness, i.e. under free-running conditions. Unexpectedly the expression of a large number of genes cycled exclusively under constant darkness. We found that cycling in most of these genes was lost in the arrhythmic Clock (Clk) mutant under light-dark conditions. Expression of periodically regulated genes is coordinated locally on chromosomes where small clusters of genes are regulated jointly. Our findings reveal that many genes involved in diverse functions are under circadian control and reveal the complexity of circadian gene expression in Drosophila.

Original languageEnglish
Pages (from-to)14048-14052
Number of pages5
JournalJournal of Biological Chemistry
Volume277
Issue number16
DOIs
Publication statusPublished - Apr 19 2002

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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