Genomewide identification of target genes of histone methyltransferase dG9a during Drosophila embryogenesis

Kouhei Shimaji, Takahiro Konishi, Shintaro Tanaka, Hideki Yoshida, Yasuko Kato, Yasuyuki Ohkawa, Tetsuya Sato, Mikita Suyama, Hiroshi Kimura, Masamitsu Yamaguchi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Post-translational modification of the histone plays important roles in epigenetic regulation of various biological processes. Among the identified histone methyltransferases (HMTases), G9a is a histone H3 Lys 9 (H3K9)-specific example active in euchromatic regions. Drosophila G9a (dG9a) has been reported to feature H3K9 dimethylation activity in vivo. Here, we show that the time required for hatching of a homozygous dG9a null mutant and heteroallelic combination of dG9a null mutants is delayed, suggesting that dG9a is at least partially responsible for progression of embryogenesis. Immunocytochemical analyses of the wild-type and the dG9a null mutant flies indicated that dG9a localizes in cytoplasm up to nuclear division cycle 7 where it is likely responsible for di-methylation of nucleosome-free H3K9. From cycles 8-11, dG9a moves into the nucleus and is responsible for di-methylating H3K9 in nucleosomes. RNA-sequence analysis utilizing early wild-type and dG9a mutant embryos showed that dG9a down-regulates expression of genes responsible for embryogenesis. RNA fluorescent in situ hybridization analysis further showed temporal and spatial expression patterns of these mRNAs did not significantly change in the dG9a mutant. These results indicate that dG9a controls transcription levels of some zygotic genes without changing temporal and spatial expression patterns of the transcripts of these genes.

Original languageEnglish
Pages (from-to)902-914
Number of pages13
JournalGenes to Cells
Volume20
Issue number11
DOIs
Publication statusPublished - Nov 2015

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Drosophila
Embryonic Development
Genes
Nucleosomes
Histones
histone methyltransferase
Cell Nucleus Division
RNA Sequence Analysis
Biological Phenomena
Post Translational Protein Processing
Fluorescence In Situ Hybridization
Epigenomics
Diptera
Methylation
Cytoplasm
Down-Regulation
Embryonic Structures
RNA
Gene Expression
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Genomewide identification of target genes of histone methyltransferase dG9a during Drosophila embryogenesis. / Shimaji, Kouhei; Konishi, Takahiro; Tanaka, Shintaro; Yoshida, Hideki; Kato, Yasuko; Ohkawa, Yasuyuki; Sato, Tetsuya; Suyama, Mikita; Kimura, Hiroshi; Yamaguchi, Masamitsu.

In: Genes to Cells, Vol. 20, No. 11, 11.2015, p. 902-914.

Research output: Contribution to journalArticle

Shimaji, K, Konishi, T, Tanaka, S, Yoshida, H, Kato, Y, Ohkawa, Y, Sato, T, Suyama, M, Kimura, H & Yamaguchi, M 2015, 'Genomewide identification of target genes of histone methyltransferase dG9a during Drosophila embryogenesis', Genes to Cells, vol. 20, no. 11, pp. 902-914. https://doi.org/10.1111/gtc.12281
Shimaji, Kouhei ; Konishi, Takahiro ; Tanaka, Shintaro ; Yoshida, Hideki ; Kato, Yasuko ; Ohkawa, Yasuyuki ; Sato, Tetsuya ; Suyama, Mikita ; Kimura, Hiroshi ; Yamaguchi, Masamitsu. / Genomewide identification of target genes of histone methyltransferase dG9a during Drosophila embryogenesis. In: Genes to Cells. 2015 ; Vol. 20, No. 11. pp. 902-914.
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