Genomic characterization of β-glucuronidase–positive escherichia coli O157:H7 producing Stx2a

Yoshitoshi Ogura, Kazuko Seto, Yo Morimoto, Keiji Nakamura, Mitsuhiko P. Sato, Yasuhiro Gotoh, Takehiko Itoh, Atsushi Toyoda, Makoto Ohnishi, Tetsuya Hayashi

Research output: Contribution to journalArticle

Abstract

Among Shiga toxin (Stx)–producing Escherichia coli (STEC) O157:H7 strains, those producing Stx2a cause more severe diseases. Atypical STEC O157:H7 strains showing a β-glucuronidase–positive phenotype (GP STEC O157:H7) have rarely been isolated from humans, mostly from persons with asymptomatic or mild infections; Stx2a-producing strains have not been reported. We isolated, from a patient with bloody diarrhea, a GP STEC O157:H7 strain (PV15-279) that produces Stx2a in addition to Stx1a and Stx2c. Genomic comparison with other STEC O157 strains revealed that PV15-279 recently emerged from the stx1a/ stx2c-positive GP STEC O157:H7 clone circulating in Japan. Major virulence genes are shared between typical (β-glucuronidase–negative) and GP STEC O157:H7 strains, and the Stx2-producing ability of PV15-279 is comparable to that of typical STEC O157:H7 strains; therefore, PV15-279 presents a virulence potential similar to that of typical STEC O157:H7. This study reveals the importance of GP O157:H7 as a source of highly pathogenic STEC clones.

Original languageEnglish
Pages (from-to)2219-2227
Number of pages9
JournalEmerging Infectious Diseases
Volume24
Issue number12
DOIs
Publication statusPublished - Dec 1 2018

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Escherichia coli O157
Virulence
Clone Cells
Shiga-Toxigenic Escherichia coli
Diarrhea
Japan
Escherichia coli
Phenotype

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Microbiology (medical)
  • Infectious Diseases

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Genomic characterization of β-glucuronidase–positive escherichia coli O157:H7 producing Stx2a. / Ogura, Yoshitoshi; Seto, Kazuko; Morimoto, Yo; Nakamura, Keiji; Sato, Mitsuhiko P.; Gotoh, Yasuhiro; Itoh, Takehiko; Toyoda, Atsushi; Ohnishi, Makoto; Hayashi, Tetsuya.

In: Emerging Infectious Diseases, Vol. 24, No. 12, 01.12.2018, p. 2219-2227.

Research output: Contribution to journalArticle

Ogura, Yoshitoshi ; Seto, Kazuko ; Morimoto, Yo ; Nakamura, Keiji ; Sato, Mitsuhiko P. ; Gotoh, Yasuhiro ; Itoh, Takehiko ; Toyoda, Atsushi ; Ohnishi, Makoto ; Hayashi, Tetsuya. / Genomic characterization of β-glucuronidase–positive escherichia coli O157:H7 producing Stx2a. In: Emerging Infectious Diseases. 2018 ; Vol. 24, No. 12. pp. 2219-2227.
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