Genomic cloning and promoter analysis of a mouse anion exchanger 3 (AE3) gene

Mitsutoshi Iwaasa, Hideki Tatewaki, Tetsuji Ohno, Kenshi Okubo, Naotaka Hamasaki, Dongchon Kang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The brain and cardiac isoforms of anion exchanger 3 (AE3) are considered to use their own promoters for their expression. However, little is known as to how the alternative transcription initiation is regulated. As a first step for elucidating the regulation, we obtained a genomic gene of mouse AE3. The 19-kbp clone contains about 6 kbp of 5′ flanking region, 23 exons, and 22 introns. We have sequenced the whole region including introns and determined the intron-exon boundaries. Six amino acids are different from those deduced from the reported mouse AE3 cDNA. We measured a promoter activity of the 5′ flanking region of the exon 1 for a brain type isoform and that of the exon C1 for a cardiac type isoform. The upstream region of the exon C1 indeed showed a promoter activity in rat cardiomyoblastic H9C2 cells, rat pheochromocyotoma PC12 cells, and human HeLa cells whereas the 5′ flanking region of the exon 1 does not in HeLa cells, suggesting that the promoter for the cardiac type is rather ubiquitously active.

Original languageEnglish
Pages (from-to)251-255
Number of pages5
JournalMitochondrial DNA
Volume13
Issue number5
DOIs
Publication statusPublished - Dec 1 2002

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Chloride-Bicarbonate Antiporters
Organism Cloning
Exons
5' Flanking Region
Introns
Genes
Protein Isoforms
HeLa Cells
PC12 Cells
Brain
Complementary DNA
Clone Cells
Amino Acids

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Genomic cloning and promoter analysis of a mouse anion exchanger 3 (AE3) gene. / Iwaasa, Mitsutoshi; Tatewaki, Hideki; Ohno, Tetsuji; Okubo, Kenshi; Hamasaki, Naotaka; Kang, Dongchon.

In: Mitochondrial DNA, Vol. 13, No. 5, 01.12.2002, p. 251-255.

Research output: Contribution to journalArticle

Iwaasa, Mitsutoshi ; Tatewaki, Hideki ; Ohno, Tetsuji ; Okubo, Kenshi ; Hamasaki, Naotaka ; Kang, Dongchon. / Genomic cloning and promoter analysis of a mouse anion exchanger 3 (AE3) gene. In: Mitochondrial DNA. 2002 ; Vol. 13, No. 5. pp. 251-255.
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