Genomic diversity of enterohemorrhagic Escherichia coli 0157 revealed by whole genome PCR scanning

Makoto Ohnishi, Jun Terajima, Ken Kurokawa, Keisuke Nakayama, Takahiro Murata, Kazumichi Tamura, Yoshitoshi Ogura, Haruo Watanabe, Tetsuya Hayashi

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

Enterohemorrhagic Escherichia coli O157 is one of the leading worldwide public health concerns, causing large outbreaks of hemorrhagic colitis as well as numerous small outbreaks and sporadic cases. The variability of restriction enzyme-digestion patterns of O157 genomes, which is widely used to distinguish strains in the molecular epidemiology of O157 infections, suggests the presence of some genomic diversity among the strains. Based on the complete genome sequence of O157 Sakai, we analyzed the whole genome structures of eight O157 strains displaying diverse Xbal-digestion patterns by a systematic PCR analysis that we have named whole genome PCR scanning. This analysis identified not only the O157-specific sequences that are highly conserved among the strains, but also revealed an unexpectedly high degree of genomic diversity. In particular, prophages, including Shiga toxin-transducing phages, exhibited extensive structural and positional diversity, implying that variation of bacteriophages is a major factor in generating genomic diversity among the O157 lineage.

Original languageEnglish
Pages (from-to)17043-17048
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number26
DOIs
Publication statusPublished - Dec 24 2002

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Enterohemorrhagic Escherichia coli
Genome
Polymerase Chain Reaction
Bacteriophages
Disease Outbreaks
Digestion
Shiga Toxin
Prophages
Escherichia coli O157
Molecular Epidemiology
Conserved Sequence
Colitis
Public Health
Enzymes
Infection

All Science Journal Classification (ASJC) codes

  • General

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Genomic diversity of enterohemorrhagic Escherichia coli 0157 revealed by whole genome PCR scanning. / Ohnishi, Makoto; Terajima, Jun; Kurokawa, Ken; Nakayama, Keisuke; Murata, Takahiro; Tamura, Kazumichi; Ogura, Yoshitoshi; Watanabe, Haruo; Hayashi, Tetsuya.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 26, 24.12.2002, p. 17043-17048.

Research output: Contribution to journalArticle

Ohnishi, Makoto ; Terajima, Jun ; Kurokawa, Ken ; Nakayama, Keisuke ; Murata, Takahiro ; Tamura, Kazumichi ; Ogura, Yoshitoshi ; Watanabe, Haruo ; Hayashi, Tetsuya. / Genomic diversity of enterohemorrhagic Escherichia coli 0157 revealed by whole genome PCR scanning. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 26. pp. 17043-17048.
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