Genomic imprinting of insulin-like growth factor-2 in infant leukemia and childhood neuroblastoma

Hiroyoshi Hattori, Akinobu Matsuzaki, Aiko Suminoe, Kenji Ihara, Mariko Eguchi, Tatsurou Tajiri, Sachiyo Suita, Eiichi Ishii, Toshiro Hara

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND. Loss of imprinting (LOI) of insulin-like growth factor-2 (IGF-2) has been implicated in the pathogenesis of certain human cancers and tumor-predisposing overgrowth disorders, such as Beckwith-Wiedemann syndrome. In a previous study, the authors revealed that certain patients with childhood acute leukemia and neuroblastoma had had rapid somatic growth after birth, suggesting the involvement of growth factor(s) in tumorigenesis. In the current study, the authors examined whether relaxation of IGF-2 imprinting occurred in infant leukemia and childhood neuroblastoma. METHODS. The genomic DNA of infant leukemia, childhood neuroblastoma, and control individuals was amplified by polymerase chain reaction (PCR). Patients who had heterozygous genotype were selected as informative cases using Apa I polymorphism in exon 9 of the IGF-2 gene. Total RNA was isolated from informative cases, followed by cDNA synthesis, cDNA was amplified by PCR, and direct sequence was performed for determining allele specific transcription. RESULTS. Twenty of 22 infant leukemia blasts and all of 16 neuroblastoma cells showed normal monoallelic expression of IGF-2 as well as 23 controls. The height and weight of two acute lymphoblastic leukemia patients with LOI were within normal ranges for Japanese children. CONCLUSIONS. The current study revealed that the imprinting status of IGF-2 was generally maintained in infant leukemia and confirmed that it was maintained in childhood neuroblastoma. The results suggest that LOI of IGF-2 does not play a major role in the carcinogenesis of these diseases or in rapid physical growth of the patients. (C) 2000 American Cancer Society.

Original languageEnglish
Pages (from-to)2372-2377
Number of pages6
JournalCancer
Volume88
Issue number10
DOIs
Publication statusPublished - May 15 2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Fingerprint

Dive into the research topics of 'Genomic imprinting of insulin-like growth factor-2 in infant leukemia and childhood neuroblastoma'. Together they form a unique fingerprint.

Cite this