Genomic organization and expression of CD8α and CD8β genes in fugu Takifugu rubripes

Hiroaki Suetake, Kyosuke Araki, Kanako Akatsu, Tomonori Somamoto, Johannes Martinus Dijkstra, Yasutoshi Yoshiura, Kiyoshi Kikuchi, Yuzuru Suzuki

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We have cloned cDNAs encoding the α and β chains of CD8 from the tiger pufferfish (fugu), Takifugu rubripes. The cDNA sequences encode a putative leader peptide, extracellular immunoglobulin variable region-like domain, stalk region, transmembrane region, and cytoplasmic tail. A protein tyrosine kinase p56lck binding motif was not found in the putative fugu CD8α cytoplasmic tail. O-linked glycosylation sites were found in the stalk of both CD8 chains, suggesting possible stalk formation. Phylogenetic analysis showed that fugu CD8α and CD8β chains cluster with other vertebrate CD8α and CD8β chains, respectively. The fugu CD8 genes comprise six exons separated by five introns. The genes are tandemly aligned 3.6 kb apart and are in the same transcription orientation. Quantitative RT-PCR analysis demonstrated that fugu CD8 is expressed predominantly in lymphoid tissues. In situ hybridization showed that fugu CD8 genes are expressed in thymocytes and lymphocytes within lymphoid organs. Molecular characterization of CD8 in fish provides the basis for development of specific antibodies to identify T-cell subsets, as well as potentially important insights into the evolution of CD8 and the adaptive immunity.

Original languageEnglish
Pages (from-to)1107-1118
Number of pages12
JournalFish and Shellfish Immunology
Volume23
Issue number5
DOIs
Publication statusPublished - Nov 1 2007

Fingerprint

Takifugu
Takifugu rubripes
genomics
Genes
gene
Complementary DNA
Immunoglobulin Variable Region
Glycosylation
T-cells
genes
Lymphocytes
Transcription
Protein Sorting Signals
immunity
peptide
Protein-Tyrosine Kinases
Introns
Fish
antibody
Exons

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

Cite this

Genomic organization and expression of CD8α and CD8β genes in fugu Takifugu rubripes. / Suetake, Hiroaki; Araki, Kyosuke; Akatsu, Kanako; Somamoto, Tomonori; Dijkstra, Johannes Martinus; Yoshiura, Yasutoshi; Kikuchi, Kiyoshi; Suzuki, Yuzuru.

In: Fish and Shellfish Immunology, Vol. 23, No. 5, 01.11.2007, p. 1107-1118.

Research output: Contribution to journalArticle

Suetake, H, Araki, K, Akatsu, K, Somamoto, T, Dijkstra, JM, Yoshiura, Y, Kikuchi, K & Suzuki, Y 2007, 'Genomic organization and expression of CD8α and CD8β genes in fugu Takifugu rubripes', Fish and Shellfish Immunology, vol. 23, no. 5, pp. 1107-1118. https://doi.org/10.1016/j.fsi.2007.05.005
Suetake, Hiroaki ; Araki, Kyosuke ; Akatsu, Kanako ; Somamoto, Tomonori ; Dijkstra, Johannes Martinus ; Yoshiura, Yasutoshi ; Kikuchi, Kiyoshi ; Suzuki, Yuzuru. / Genomic organization and expression of CD8α and CD8β genes in fugu Takifugu rubripes. In: Fish and Shellfish Immunology. 2007 ; Vol. 23, No. 5. pp. 1107-1118.
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