Genomic organization of the human Y-box protein (YB-1) gene

Satoshi Toh, Takanori Nakamura, Takefumi Ohga, Koji Koike, Takeshi Uchiumi, Morimasa Wada, Michihiko Kuwano, Kimitoshi Kohno

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The human Y-box protein (YB-1) is a member of a family of DNA-binding proteins contain a highly conserved cold shock domain. The genomic organization of the human YB-1 gene was determined from five overlapping genomic clones that encompassed all exons of the gene. Sequence analysis of these clones revealed that human YB-1 spans approximately 19 kb of genomic DNA and contains eight exons. The cold shock domain is encoded by exons 1-5. Both exon-splitting and codon-splitting in the region of the gene encoding the cold shock domain are similar to those in the corresponding region of another Y-box binding protein, dbpA. Exon-intron structures and nucleotide sequences of the regions encoding the N-terminal and C-terminal domains of the two proteins differ markedly between YB-1 and dbpA. These observations suggest that YB-1 and dbpA arose by duplication of a common ancestral gene encoding all these domains.

Original languageEnglish
Pages (from-to)93-97
Number of pages5
JournalGene
Volume206
Issue number1
DOIs
Publication statusPublished - Jan 5 1998

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Exons
Shock
Genes
Clone Cells
DNA-Binding Proteins
Codon
Introns
Sequence Analysis
Carrier Proteins
human YBX1 protein
DNA

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Toh, S., Nakamura, T., Ohga, T., Koike, K., Uchiumi, T., Wada, M., ... Kohno, K. (1998). Genomic organization of the human Y-box protein (YB-1) gene. Gene, 206(1), 93-97. https://doi.org/10.1016/S0378-1119(97)00570-2

Genomic organization of the human Y-box protein (YB-1) gene. / Toh, Satoshi; Nakamura, Takanori; Ohga, Takefumi; Koike, Koji; Uchiumi, Takeshi; Wada, Morimasa; Kuwano, Michihiko; Kohno, Kimitoshi.

In: Gene, Vol. 206, No. 1, 05.01.1998, p. 93-97.

Research output: Contribution to journalArticle

Toh, S, Nakamura, T, Ohga, T, Koike, K, Uchiumi, T, Wada, M, Kuwano, M & Kohno, K 1998, 'Genomic organization of the human Y-box protein (YB-1) gene', Gene, vol. 206, no. 1, pp. 93-97. https://doi.org/10.1016/S0378-1119(97)00570-2
Toh, Satoshi ; Nakamura, Takanori ; Ohga, Takefumi ; Koike, Koji ; Uchiumi, Takeshi ; Wada, Morimasa ; Kuwano, Michihiko ; Kohno, Kimitoshi. / Genomic organization of the human Y-box protein (YB-1) gene. In: Gene. 1998 ; Vol. 206, No. 1. pp. 93-97.
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