Genomic organization, sequence and chromosomal localization of the mouse Tbr2 gene and a comparative study with Tbr1

Masaya Ueno, Naoki Kimura, Kinichi Nakashima, Fumiko Saito-Ohara, Johji Inazawa, Tetsuya Taga

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Members of the T-box family are known to play critical roles in the embryonic development of most animal species. Recently, we have isolated its new mammalian member, Tbr2, from mouse embryonic brain. We have also shown that the expression patterns of Tbr2 and the closely related Tbr1 appear to be reciprocal in the developing brain; Tbr2 is expressed in mesencephalon and rhombencephalon, but expression of Tbr1 is restricted to telencephalon. To investigate possible structural and functional relationships of Tbr2 and other T-box containing genes, we analyzed genomic organization of the murine Tbr2 gene. The Tbr2 gene is composed of six exons (1353, 155, 122, 159, 62 and 1035 bp), and five introns (920, 643, 602, 85 and 2036 bp). This exon/intron organization is very similar to that of Tbr1. We also analyzed the 3.9 kb sequence of the 5' promoter region flanking the Tbr2 gene and the corresponding region of the Tbr1 gene. The sites for Brn-2 and Tst-1 were found in the promoter of Tbr2 but not Tbr1. On the contrary, there were eight HNF-3β binding sites in the Tbr1 gene promoter but only three in the Tbr2 promoter. The differential presence of putative binding sites for these brain-specific transcription factors may explain the reciprocal expression of Tbr1 and Tbr2. Furthermore, a single chromosomal locus for mouse Tbr2 was assigned to 9F3 by fluorescence in-situ hybridization. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)29-35
Number of pages7
JournalGene
Volume254
Issue number1-2
DOIs
Publication statusPublished - Aug 22 2000
Externally publishedYes

Fingerprint

Genes
Introns
Exons
Brain
Binding Sites
Telencephalon
Rhombencephalon
5' Flanking Region
Mesencephalon
Fluorescence In Situ Hybridization
Genetic Promoter Regions
Embryonic Development
Transcription Factors

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Genomic organization, sequence and chromosomal localization of the mouse Tbr2 gene and a comparative study with Tbr1. / Ueno, Masaya; Kimura, Naoki; Nakashima, Kinichi; Saito-Ohara, Fumiko; Inazawa, Johji; Taga, Tetsuya.

In: Gene, Vol. 254, No. 1-2, 22.08.2000, p. 29-35.

Research output: Contribution to journalArticle

Ueno, Masaya ; Kimura, Naoki ; Nakashima, Kinichi ; Saito-Ohara, Fumiko ; Inazawa, Johji ; Taga, Tetsuya. / Genomic organization, sequence and chromosomal localization of the mouse Tbr2 gene and a comparative study with Tbr1. In: Gene. 2000 ; Vol. 254, No. 1-2. pp. 29-35.
@article{8318d53aa1ed4f7cbe40192d6ed8ce7b,
title = "Genomic organization, sequence and chromosomal localization of the mouse Tbr2 gene and a comparative study with Tbr1",
abstract = "Members of the T-box family are known to play critical roles in the embryonic development of most animal species. Recently, we have isolated its new mammalian member, Tbr2, from mouse embryonic brain. We have also shown that the expression patterns of Tbr2 and the closely related Tbr1 appear to be reciprocal in the developing brain; Tbr2 is expressed in mesencephalon and rhombencephalon, but expression of Tbr1 is restricted to telencephalon. To investigate possible structural and functional relationships of Tbr2 and other T-box containing genes, we analyzed genomic organization of the murine Tbr2 gene. The Tbr2 gene is composed of six exons (1353, 155, 122, 159, 62 and 1035 bp), and five introns (920, 643, 602, 85 and 2036 bp). This exon/intron organization is very similar to that of Tbr1. We also analyzed the 3.9 kb sequence of the 5' promoter region flanking the Tbr2 gene and the corresponding region of the Tbr1 gene. The sites for Brn-2 and Tst-1 were found in the promoter of Tbr2 but not Tbr1. On the contrary, there were eight HNF-3β binding sites in the Tbr1 gene promoter but only three in the Tbr2 promoter. The differential presence of putative binding sites for these brain-specific transcription factors may explain the reciprocal expression of Tbr1 and Tbr2. Furthermore, a single chromosomal locus for mouse Tbr2 was assigned to 9F3 by fluorescence in-situ hybridization. (C) 2000 Elsevier Science B.V.",
author = "Masaya Ueno and Naoki Kimura and Kinichi Nakashima and Fumiko Saito-Ohara and Johji Inazawa and Tetsuya Taga",
year = "2000",
month = "8",
day = "22",
doi = "10.1016/S0378-1119(00)00290-0",
language = "English",
volume = "254",
pages = "29--35",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Genomic organization, sequence and chromosomal localization of the mouse Tbr2 gene and a comparative study with Tbr1

AU - Ueno, Masaya

AU - Kimura, Naoki

AU - Nakashima, Kinichi

AU - Saito-Ohara, Fumiko

AU - Inazawa, Johji

AU - Taga, Tetsuya

PY - 2000/8/22

Y1 - 2000/8/22

N2 - Members of the T-box family are known to play critical roles in the embryonic development of most animal species. Recently, we have isolated its new mammalian member, Tbr2, from mouse embryonic brain. We have also shown that the expression patterns of Tbr2 and the closely related Tbr1 appear to be reciprocal in the developing brain; Tbr2 is expressed in mesencephalon and rhombencephalon, but expression of Tbr1 is restricted to telencephalon. To investigate possible structural and functional relationships of Tbr2 and other T-box containing genes, we analyzed genomic organization of the murine Tbr2 gene. The Tbr2 gene is composed of six exons (1353, 155, 122, 159, 62 and 1035 bp), and five introns (920, 643, 602, 85 and 2036 bp). This exon/intron organization is very similar to that of Tbr1. We also analyzed the 3.9 kb sequence of the 5' promoter region flanking the Tbr2 gene and the corresponding region of the Tbr1 gene. The sites for Brn-2 and Tst-1 were found in the promoter of Tbr2 but not Tbr1. On the contrary, there were eight HNF-3β binding sites in the Tbr1 gene promoter but only three in the Tbr2 promoter. The differential presence of putative binding sites for these brain-specific transcription factors may explain the reciprocal expression of Tbr1 and Tbr2. Furthermore, a single chromosomal locus for mouse Tbr2 was assigned to 9F3 by fluorescence in-situ hybridization. (C) 2000 Elsevier Science B.V.

AB - Members of the T-box family are known to play critical roles in the embryonic development of most animal species. Recently, we have isolated its new mammalian member, Tbr2, from mouse embryonic brain. We have also shown that the expression patterns of Tbr2 and the closely related Tbr1 appear to be reciprocal in the developing brain; Tbr2 is expressed in mesencephalon and rhombencephalon, but expression of Tbr1 is restricted to telencephalon. To investigate possible structural and functional relationships of Tbr2 and other T-box containing genes, we analyzed genomic organization of the murine Tbr2 gene. The Tbr2 gene is composed of six exons (1353, 155, 122, 159, 62 and 1035 bp), and five introns (920, 643, 602, 85 and 2036 bp). This exon/intron organization is very similar to that of Tbr1. We also analyzed the 3.9 kb sequence of the 5' promoter region flanking the Tbr2 gene and the corresponding region of the Tbr1 gene. The sites for Brn-2 and Tst-1 were found in the promoter of Tbr2 but not Tbr1. On the contrary, there were eight HNF-3β binding sites in the Tbr1 gene promoter but only three in the Tbr2 promoter. The differential presence of putative binding sites for these brain-specific transcription factors may explain the reciprocal expression of Tbr1 and Tbr2. Furthermore, a single chromosomal locus for mouse Tbr2 was assigned to 9F3 by fluorescence in-situ hybridization. (C) 2000 Elsevier Science B.V.

UR - http://www.scopus.com/inward/record.url?scp=0034702884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034702884&partnerID=8YFLogxK

U2 - 10.1016/S0378-1119(00)00290-0

DO - 10.1016/S0378-1119(00)00290-0

M3 - Article

C2 - 10974533

AN - SCOPUS:0034702884

VL - 254

SP - 29

EP - 35

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1-2

ER -