Genomic structure and 5′ regulatory regions of the let-23 gene in the nematode C. Elegans

Tomoe Sakai, Makoto Koga, Yasumi Ohshima

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The let-23 gene in the nematode Caenorhabditis elegans encodes a receptor tyrosine kinase and is necessary for the induction of a vulva, survival past the L1 stage, hermaphrodite fertility and for male spicule development. We sequenced the entire let-23 genomic region of over 12 kb. The 5′ end of the let-23 mRNA was mapped by sequencing polymerase chain reaction products, and two mRNAs were found which had alternative exons and were probably initiated at different sites. One transcript was trans-spliced to SL1. Expression of either cDNA rescued a let-23 vulvaless mutation in germline transformation. These results suggest that the let-23 gene encodes two proteins of 1323 or 1335 amino acid residues. We prepared various 5′ deletion constructs of the let-23 gene, and examined their rescue activities for a let-23 lethal or vulvaless mutation. The results revealed that two regions were required for let-23 expression, one for larval survival and the other for vulva formation. We also cloned and analyzed a let-23 homologue from Caenorhabditis vulgaris. It can encode two proteins of 77% amino acid residue identity with the Let-23 proteins. The 12 kb fragment carrying the C. vulgaris gene rescued the let-23 vulvaless mutation in C. elegans. Seventeen sequences highly conserved between the two species were identified in the 5′ upstream region or within an intron. Three of these sequences are contained in the two regions required for let-23 expression, suggesting that they are cis-acting elements for let-23 expression.

Original languageEnglish
Pages (from-to)548-555
Number of pages8
JournalJournal of Molecular Biology
Volume256
Issue number3
DOIs
Publication statusPublished - Mar 1 1996

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Nucleic Acid Regulatory Sequences
Vulva
Caenorhabditis elegans
Genes
Caenorhabditis
Amino Acids
Messenger RNA
Mutation
Proteins
Germ-Line Mutation
Conserved Sequence
Receptor Protein-Tyrosine Kinases
Introns
Fertility
Exons
Complementary DNA
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Genomic structure and 5′ regulatory regions of the let-23 gene in the nematode C. Elegans. / Sakai, Tomoe; Koga, Makoto; Ohshima, Yasumi.

In: Journal of Molecular Biology, Vol. 256, No. 3, 01.03.1996, p. 548-555.

Research output: Contribution to journalArticle

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