Genomic structure and chromosome location of the human mutT homologue gene MTH1 encoding 8-Oxo-dGTPase for prevention of A: T to C: G transversion

Masato Furuichi, Michihiro C. Yoshida, Hisanobu Oda, Tatsurou Tajiri, Yusaku Nakabeppu, Teruhisa Tsuzuki, Mutsuo Sekiguchi

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

8-Oxo-dGTP (8-oxo-7,8-dihydrodeoxyguanosine triphosphate) is produced by active oxygen species in the nucleotide pool of the cell and can be incorporated into cellular DNA. Human cells contain enzyme activity that hydrolyzes 8-oxo-dGTP to 8-oxo-dGMP, thereby preventing occurrence of mutations, caused by misincorporation. When the cDNA for human 8-oxo-dGTPase was expressed in Escherichia coli mutT-mutant cells devoid of self 8-oxo-dGTPase activity, the elevated level of spontaneous A:T to C:G mutation frequency reverted to normal. We isolated the genomic sequence encoding the enzyme and named the gene MTH1 (for mutT human homologue). This gene is composed of at least 4 exons, spans approximately 9 kb, and is located on human chromosome 7p22.

Original languageEnglish
Pages (from-to)485-490
Number of pages6
JournalGenomics
Volume24
Issue number3
DOIs
Publication statusPublished - Jan 1 1994

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Chromosome Structures
Human Chromosomes
Genes
Mutation Rate
Enzymes
Exons
Reactive Oxygen Species
Nucleotides
Complementary DNA
Escherichia coli
Mutation
DNA
8-oxodGTPase

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Genomic structure and chromosome location of the human mutT homologue gene MTH1 encoding 8-Oxo-dGTPase for prevention of A : T to C: G transversion. / Furuichi, Masato; Yoshida, Michihiro C.; Oda, Hisanobu; Tajiri, Tatsurou; Nakabeppu, Yusaku; Tsuzuki, Teruhisa; Sekiguchi, Mutsuo.

In: Genomics, Vol. 24, No. 3, 01.01.1994, p. 485-490.

Research output: Contribution to journalArticle

Furuichi, Masato ; Yoshida, Michihiro C. ; Oda, Hisanobu ; Tajiri, Tatsurou ; Nakabeppu, Yusaku ; Tsuzuki, Teruhisa ; Sekiguchi, Mutsuo. / Genomic structure and chromosome location of the human mutT homologue gene MTH1 encoding 8-Oxo-dGTPase for prevention of A : T to C: G transversion. In: Genomics. 1994 ; Vol. 24, No. 3. pp. 485-490.
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