Abstract
Although Streptococcus suis has attracted public attention as a major swine and human pathogen, this bacterium has also been isolated from other animals, including ruminants. However, recent taxonomic studies revealed the existence of other species that were previously identified as S. suis, and some of these isolates were reclassified as the novel species Streptococcus ruminantium. In Japan, biochemically identified S. suis is frequently isolated from diseased ruminants; however, such isolates have not yet been identified accurately, and their aetiological importance in ruminants is unclear. Therefore, to understand the importance of S. suis and S. suis-like bacteria in ruminants, we reclassified S. suis isolates from ruminants according to the updated classification and investigated their genetic diversity. Although both S. suis and S. ruminantium were isolated from healthy and diseased ruminants, most of the isolates from diseased animals were S. ruminantium, implying that S. ruminantium is more likely to be associated with ruminant disease than S. suis. However, the ruminant S. suis and S. ruminantium isolates from diseased animals were classified into diverse genotypes rather than belonging to certain clonal groups. Genome sequence analysis of 20 S. ruminantium isolates provided information about the antibiotic resistance, potential virulence, and serological diversity of this species. We further developed an S. ruminantium-specific PCR assay to aid in the identification of this bacterium. The information obtained and the method established in this study will contribute to the accurate diagnosis of ruminant streptococcal infections.
| Original language | English |
|---|---|
| Article number | 94 |
| Journal | Veterinary Research |
| Volume | 50 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Nov 14 2019 |
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All Science Journal Classification (ASJC) codes
- veterinary(all)
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Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants. / Okura, Masatoshi; Maruyama, Fumito; Ota, Atsushi; Tanaka, Takeshi; Matoba, Yohei; Osawa, Aya; Sadaat, Sayed Mushtaq; Osaki, Makoto; Toyoda, Atsushi; Ogura, Yoshitoshi; Hayashi, Tetsuya; Takamatsu, Daisuke.
In: Veterinary Research, Vol. 50, No. 1, 94, 14.11.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants
AU - Okura, Masatoshi
AU - Maruyama, Fumito
AU - Ota, Atsushi
AU - Tanaka, Takeshi
AU - Matoba, Yohei
AU - Osawa, Aya
AU - Sadaat, Sayed Mushtaq
AU - Osaki, Makoto
AU - Toyoda, Atsushi
AU - Ogura, Yoshitoshi
AU - Hayashi, Tetsuya
AU - Takamatsu, Daisuke
PY - 2019/11/14
Y1 - 2019/11/14
N2 - Although Streptococcus suis has attracted public attention as a major swine and human pathogen, this bacterium has also been isolated from other animals, including ruminants. However, recent taxonomic studies revealed the existence of other species that were previously identified as S. suis, and some of these isolates were reclassified as the novel species Streptococcus ruminantium. In Japan, biochemically identified S. suis is frequently isolated from diseased ruminants; however, such isolates have not yet been identified accurately, and their aetiological importance in ruminants is unclear. Therefore, to understand the importance of S. suis and S. suis-like bacteria in ruminants, we reclassified S. suis isolates from ruminants according to the updated classification and investigated their genetic diversity. Although both S. suis and S. ruminantium were isolated from healthy and diseased ruminants, most of the isolates from diseased animals were S. ruminantium, implying that S. ruminantium is more likely to be associated with ruminant disease than S. suis. However, the ruminant S. suis and S. ruminantium isolates from diseased animals were classified into diverse genotypes rather than belonging to certain clonal groups. Genome sequence analysis of 20 S. ruminantium isolates provided information about the antibiotic resistance, potential virulence, and serological diversity of this species. We further developed an S. ruminantium-specific PCR assay to aid in the identification of this bacterium. The information obtained and the method established in this study will contribute to the accurate diagnosis of ruminant streptococcal infections.
AB - Although Streptococcus suis has attracted public attention as a major swine and human pathogen, this bacterium has also been isolated from other animals, including ruminants. However, recent taxonomic studies revealed the existence of other species that were previously identified as S. suis, and some of these isolates were reclassified as the novel species Streptococcus ruminantium. In Japan, biochemically identified S. suis is frequently isolated from diseased ruminants; however, such isolates have not yet been identified accurately, and their aetiological importance in ruminants is unclear. Therefore, to understand the importance of S. suis and S. suis-like bacteria in ruminants, we reclassified S. suis isolates from ruminants according to the updated classification and investigated their genetic diversity. Although both S. suis and S. ruminantium were isolated from healthy and diseased ruminants, most of the isolates from diseased animals were S. ruminantium, implying that S. ruminantium is more likely to be associated with ruminant disease than S. suis. However, the ruminant S. suis and S. ruminantium isolates from diseased animals were classified into diverse genotypes rather than belonging to certain clonal groups. Genome sequence analysis of 20 S. ruminantium isolates provided information about the antibiotic resistance, potential virulence, and serological diversity of this species. We further developed an S. ruminantium-specific PCR assay to aid in the identification of this bacterium. The information obtained and the method established in this study will contribute to the accurate diagnosis of ruminant streptococcal infections.
UR - http://www.scopus.com/inward/record.url?scp=85075114086&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85075114086&partnerID=8YFLogxK
U2 - 10.1186/s13567-019-0708-1
DO - 10.1186/s13567-019-0708-1
M3 - Article
C2 - 31727180
AN - SCOPUS:85075114086
VL - 50
JO - Annales de Recherches Veterinaires
JF - Annales de Recherches Veterinaires
SN - 0928-4249
IS - 1
M1 - 94
ER -