Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants

Masatoshi Okura, Fumito Maruyama, Atsushi Ota, Takeshi Tanaka, Yohei Matoba, Aya Osawa, Sayed Mushtaq Sadaat, Makoto Osaki, Atsushi Toyoda, Yoshitoshi Ogura, Tetsuya Hayashi, Daisuke Takamatsu

Research output: Contribution to journalArticle

Abstract

Although Streptococcus suis has attracted public attention as a major swine and human pathogen, this bacterium has also been isolated from other animals, including ruminants. However, recent taxonomic studies revealed the existence of other species that were previously identified as S. suis, and some of these isolates were reclassified as the novel species Streptococcus ruminantium. In Japan, biochemically identified S. suis is frequently isolated from diseased ruminants; however, such isolates have not yet been identified accurately, and their aetiological importance in ruminants is unclear. Therefore, to understand the importance of S. suis and S. suis-like bacteria in ruminants, we reclassified S. suis isolates from ruminants according to the updated classification and investigated their genetic diversity. Although both S. suis and S. ruminantium were isolated from healthy and diseased ruminants, most of the isolates from diseased animals were S. ruminantium, implying that S. ruminantium is more likely to be associated with ruminant disease than S. suis. However, the ruminant S. suis and S. ruminantium isolates from diseased animals were classified into diverse genotypes rather than belonging to certain clonal groups. Genome sequence analysis of 20 S. ruminantium isolates provided information about the antibiotic resistance, potential virulence, and serological diversity of this species. We further developed an S. ruminantium-specific PCR assay to aid in the identification of this bacterium. The information obtained and the method established in this study will contribute to the accurate diagnosis of ruminant streptococcal infections.

Original languageEnglish
Article number94
JournalVeterinary Research
Volume50
Issue number1
DOIs
Publication statusPublished - Nov 14 2019

Fingerprint

Streptococcus suis
Streptococcus
Ruminants
ruminants
Bacteria
genetic variation
bacteria
Animal Diseases
Streptococcal Infections
animals

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants. / Okura, Masatoshi; Maruyama, Fumito; Ota, Atsushi; Tanaka, Takeshi; Matoba, Yohei; Osawa, Aya; Sadaat, Sayed Mushtaq; Osaki, Makoto; Toyoda, Atsushi; Ogura, Yoshitoshi; Hayashi, Tetsuya; Takamatsu, Daisuke.

In: Veterinary Research, Vol. 50, No. 1, 94, 14.11.2019.

Research output: Contribution to journalArticle

Okura, M, Maruyama, F, Ota, A, Tanaka, T, Matoba, Y, Osawa, A, Sadaat, SM, Osaki, M, Toyoda, A, Ogura, Y, Hayashi, T & Takamatsu, D 2019, 'Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants', Veterinary Research, vol. 50, no. 1, 94. https://doi.org/10.1186/s13567-019-0708-1
Okura, Masatoshi ; Maruyama, Fumito ; Ota, Atsushi ; Tanaka, Takeshi ; Matoba, Yohei ; Osawa, Aya ; Sadaat, Sayed Mushtaq ; Osaki, Makoto ; Toyoda, Atsushi ; Ogura, Yoshitoshi ; Hayashi, Tetsuya ; Takamatsu, Daisuke. / Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants. In: Veterinary Research. 2019 ; Vol. 50, No. 1.
@article{d27e3dfb66b549558928238455e46e57,
title = "Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants",
abstract = "Although Streptococcus suis has attracted public attention as a major swine and human pathogen, this bacterium has also been isolated from other animals, including ruminants. However, recent taxonomic studies revealed the existence of other species that were previously identified as S. suis, and some of these isolates were reclassified as the novel species Streptococcus ruminantium. In Japan, biochemically identified S. suis is frequently isolated from diseased ruminants; however, such isolates have not yet been identified accurately, and their aetiological importance in ruminants is unclear. Therefore, to understand the importance of S. suis and S. suis-like bacteria in ruminants, we reclassified S. suis isolates from ruminants according to the updated classification and investigated their genetic diversity. Although both S. suis and S. ruminantium were isolated from healthy and diseased ruminants, most of the isolates from diseased animals were S. ruminantium, implying that S. ruminantium is more likely to be associated with ruminant disease than S. suis. However, the ruminant S. suis and S. ruminantium isolates from diseased animals were classified into diverse genotypes rather than belonging to certain clonal groups. Genome sequence analysis of 20 S. ruminantium isolates provided information about the antibiotic resistance, potential virulence, and serological diversity of this species. We further developed an S. ruminantium-specific PCR assay to aid in the identification of this bacterium. The information obtained and the method established in this study will contribute to the accurate diagnosis of ruminant streptococcal infections.",
author = "Masatoshi Okura and Fumito Maruyama and Atsushi Ota and Takeshi Tanaka and Yohei Matoba and Aya Osawa and Sadaat, {Sayed Mushtaq} and Makoto Osaki and Atsushi Toyoda and Yoshitoshi Ogura and Tetsuya Hayashi and Daisuke Takamatsu",
year = "2019",
month = "11",
day = "14",
doi = "10.1186/s13567-019-0708-1",
language = "English",
volume = "50",
journal = "Annales de Recherches Veterinaires",
issn = "0928-4249",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants

AU - Okura, Masatoshi

AU - Maruyama, Fumito

AU - Ota, Atsushi

AU - Tanaka, Takeshi

AU - Matoba, Yohei

AU - Osawa, Aya

AU - Sadaat, Sayed Mushtaq

AU - Osaki, Makoto

AU - Toyoda, Atsushi

AU - Ogura, Yoshitoshi

AU - Hayashi, Tetsuya

AU - Takamatsu, Daisuke

PY - 2019/11/14

Y1 - 2019/11/14

N2 - Although Streptococcus suis has attracted public attention as a major swine and human pathogen, this bacterium has also been isolated from other animals, including ruminants. However, recent taxonomic studies revealed the existence of other species that were previously identified as S. suis, and some of these isolates were reclassified as the novel species Streptococcus ruminantium. In Japan, biochemically identified S. suis is frequently isolated from diseased ruminants; however, such isolates have not yet been identified accurately, and their aetiological importance in ruminants is unclear. Therefore, to understand the importance of S. suis and S. suis-like bacteria in ruminants, we reclassified S. suis isolates from ruminants according to the updated classification and investigated their genetic diversity. Although both S. suis and S. ruminantium were isolated from healthy and diseased ruminants, most of the isolates from diseased animals were S. ruminantium, implying that S. ruminantium is more likely to be associated with ruminant disease than S. suis. However, the ruminant S. suis and S. ruminantium isolates from diseased animals were classified into diverse genotypes rather than belonging to certain clonal groups. Genome sequence analysis of 20 S. ruminantium isolates provided information about the antibiotic resistance, potential virulence, and serological diversity of this species. We further developed an S. ruminantium-specific PCR assay to aid in the identification of this bacterium. The information obtained and the method established in this study will contribute to the accurate diagnosis of ruminant streptococcal infections.

AB - Although Streptococcus suis has attracted public attention as a major swine and human pathogen, this bacterium has also been isolated from other animals, including ruminants. However, recent taxonomic studies revealed the existence of other species that were previously identified as S. suis, and some of these isolates were reclassified as the novel species Streptococcus ruminantium. In Japan, biochemically identified S. suis is frequently isolated from diseased ruminants; however, such isolates have not yet been identified accurately, and their aetiological importance in ruminants is unclear. Therefore, to understand the importance of S. suis and S. suis-like bacteria in ruminants, we reclassified S. suis isolates from ruminants according to the updated classification and investigated their genetic diversity. Although both S. suis and S. ruminantium were isolated from healthy and diseased ruminants, most of the isolates from diseased animals were S. ruminantium, implying that S. ruminantium is more likely to be associated with ruminant disease than S. suis. However, the ruminant S. suis and S. ruminantium isolates from diseased animals were classified into diverse genotypes rather than belonging to certain clonal groups. Genome sequence analysis of 20 S. ruminantium isolates provided information about the antibiotic resistance, potential virulence, and serological diversity of this species. We further developed an S. ruminantium-specific PCR assay to aid in the identification of this bacterium. The information obtained and the method established in this study will contribute to the accurate diagnosis of ruminant streptococcal infections.

UR - http://www.scopus.com/inward/record.url?scp=85075114086&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85075114086&partnerID=8YFLogxK

U2 - 10.1186/s13567-019-0708-1

DO - 10.1186/s13567-019-0708-1

M3 - Article

C2 - 31727180

AN - SCOPUS:85075114086

VL - 50

JO - Annales de Recherches Veterinaires

JF - Annales de Recherches Veterinaires

SN - 0928-4249

IS - 1

M1 - 94

ER -