Genotyping of Erwinia carotovora subsp. carotovora strains from Asia based on recA gene restriction fragment length polymorphisms

Sang Tae Seo, Naruto Furuya, Minoru Takeshita, Yoichi Takanami

Research output: Contribution to journalArticle

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Abstract

Genetic diversity of a collection of 92 strains of Erwinia carotovora subsp. carotovora (Ecc) isolated from various host, plants in Asian countries (Japan, Korea and Thailand) was assessed by means of PCR-RFLP of recombinase A gene (recA). Ten RFLP groups were obtained on the basis of restriction analysis of recA gene fragments with four restriction endonucleases (Hind III, Alu I, Dde I and Tas I). Most of Asian strains (71 out of 92 strains) belonged to RFLP groups 1, 2 and 3. When the RFLP groups were compared with the phenotypic groups and hosts of origin, there were found some relationships. RFLP groups 2 and 3 coincided with phenotypic groups A and B, respectively. RFLP group 4 contained only isolates from mulberry trees, indicating that the mulberry strains are in a distinct group in Ecc. The results of this study will facilitate further understanding of the population structure of Ecc in Asia.

Original languageEnglish
Pages (from-to)7-12
Number of pages6
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume47
Issue number1
Publication statusPublished - Oct 2002

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Pectobacterium carotovorum
Pectobacterium carotovorum subsp. carotovorum
Recombinases
Restriction Fragment Length Polymorphisms
genotyping
restriction fragment length polymorphism
Genes
Morus
genes
mulberries
DNA Restriction Enzymes
restriction endonucleases
Thailand
Korea
Korean Peninsula
Japan
population structure
host plants
Polymerase Chain Reaction
genetic variation

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)

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Genotyping of Erwinia carotovora subsp. carotovora strains from Asia based on recA gene restriction fragment length polymorphisms. / Seo, Sang Tae; Furuya, Naruto; Takeshita, Minoru; Takanami, Yoichi.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 47, No. 1, 10.2002, p. 7-12.

Research output: Contribution to journalArticle

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