Geographic distribution of fluoroquinolone-resistant Escherichia coli strains in Asia

Yujiro Uchida, Tomomi Mochimaru, Yuiko Morokuma, Makiko Kiyosuke, Masako Fujise, Fujiko Eto, Yukiko Harada, Masako Kadowaki, Nobuyuki Shimono, Dongchon Kang

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Fluoroquinolone (FQ) resistance is usually caused by point mutations within the quinolone resistance-determining regions (QRDRs) of gyrA, gyrB, parC and/or parE. However, little is known about the worldwide increase in FQ-resistant Escherichia coli or, more specifically, about the geographical distribution of QRDR mutations and the clonal spread of isolates. In this study, we analysed 68 FQ-resistant E. coli isolates from eight Asian countries using QRDR amino acid mutation patterns and examined their susceptibility to FQs. Of the isolates, 38% had mutations at S83 and D87 of GyrA and S80 of ParC (MM/-/M-/-) and 34% had mutations at S83 and D87 of GyrA, S80 of ParC and S458 of ParE (MM/-/M-/M). MIC50 values (minimum inhibitory concentrations for 50% of the isolates) for isolates with at least mutation at S458 of ParE for ciprofloxacin and prulifloxacin were relatively higher than MIC50 values of isolates without this mutation. Based on their geographic distribution and the QRDR mutation patterns, the isolates were divided into a common type in which the organisms were isolated from three or more countries, and a local type in which the isolates were from one or two countries. Mutation types at S83L and D87N in GyrA and S80I in ParC with no or another site in the QRDR were the most frequent among the FQ-resistant isolates, especially among the common type. Gene typing indicated that isolates in the common type were not similar between countries. These data suggest that the increase in FQ-resistant E. coli strains is mainly generated by mutations in the QRDR in each geographical area rather than through intercontinental spread.

Original languageEnglish
Pages (from-to)387-391
Number of pages5
JournalInternational Journal of Antimicrobial Agents
Volume35
Issue number4
DOIs
Publication statusPublished - Apr 1 2010

Fingerprint

Fluoroquinolones
Quinolones
Escherichia coli
Mutation
Microbial Sensitivity Tests
Ciprofloxacin
Point Mutation
Inhibitory Concentration 50
Amino Acids

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

Geographic distribution of fluoroquinolone-resistant Escherichia coli strains in Asia. / Uchida, Yujiro; Mochimaru, Tomomi; Morokuma, Yuiko; Kiyosuke, Makiko; Fujise, Masako; Eto, Fujiko; Harada, Yukiko; Kadowaki, Masako; Shimono, Nobuyuki; Kang, Dongchon.

In: International Journal of Antimicrobial Agents, Vol. 35, No. 4, 01.04.2010, p. 387-391.

Research output: Contribution to journalArticle

Uchida, Y, Mochimaru, T, Morokuma, Y, Kiyosuke, M, Fujise, M, Eto, F, Harada, Y, Kadowaki, M, Shimono, N & Kang, D 2010, 'Geographic distribution of fluoroquinolone-resistant Escherichia coli strains in Asia', International Journal of Antimicrobial Agents, vol. 35, no. 4, pp. 387-391. https://doi.org/10.1016/j.ijantimicag.2009.12.005
Uchida, Yujiro ; Mochimaru, Tomomi ; Morokuma, Yuiko ; Kiyosuke, Makiko ; Fujise, Masako ; Eto, Fujiko ; Harada, Yukiko ; Kadowaki, Masako ; Shimono, Nobuyuki ; Kang, Dongchon. / Geographic distribution of fluoroquinolone-resistant Escherichia coli strains in Asia. In: International Journal of Antimicrobial Agents. 2010 ; Vol. 35, No. 4. pp. 387-391.
@article{543df94e58a8433fb972a556c3b5466f,
title = "Geographic distribution of fluoroquinolone-resistant Escherichia coli strains in Asia",
abstract = "Fluoroquinolone (FQ) resistance is usually caused by point mutations within the quinolone resistance-determining regions (QRDRs) of gyrA, gyrB, parC and/or parE. However, little is known about the worldwide increase in FQ-resistant Escherichia coli or, more specifically, about the geographical distribution of QRDR mutations and the clonal spread of isolates. In this study, we analysed 68 FQ-resistant E. coli isolates from eight Asian countries using QRDR amino acid mutation patterns and examined their susceptibility to FQs. Of the isolates, 38{\%} had mutations at S83 and D87 of GyrA and S80 of ParC (MM/-/M-/-) and 34{\%} had mutations at S83 and D87 of GyrA, S80 of ParC and S458 of ParE (MM/-/M-/M). MIC50 values (minimum inhibitory concentrations for 50{\%} of the isolates) for isolates with at least mutation at S458 of ParE for ciprofloxacin and prulifloxacin were relatively higher than MIC50 values of isolates without this mutation. Based on their geographic distribution and the QRDR mutation patterns, the isolates were divided into a common type in which the organisms were isolated from three or more countries, and a local type in which the isolates were from one or two countries. Mutation types at S83L and D87N in GyrA and S80I in ParC with no or another site in the QRDR were the most frequent among the FQ-resistant isolates, especially among the common type. Gene typing indicated that isolates in the common type were not similar between countries. These data suggest that the increase in FQ-resistant E. coli strains is mainly generated by mutations in the QRDR in each geographical area rather than through intercontinental spread.",
author = "Yujiro Uchida and Tomomi Mochimaru and Yuiko Morokuma and Makiko Kiyosuke and Masako Fujise and Fujiko Eto and Yukiko Harada and Masako Kadowaki and Nobuyuki Shimono and Dongchon Kang",
year = "2010",
month = "4",
day = "1",
doi = "10.1016/j.ijantimicag.2009.12.005",
language = "English",
volume = "35",
pages = "387--391",
journal = "International Journal of Antimicrobial Agents",
issn = "0924-8579",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Geographic distribution of fluoroquinolone-resistant Escherichia coli strains in Asia

AU - Uchida, Yujiro

AU - Mochimaru, Tomomi

AU - Morokuma, Yuiko

AU - Kiyosuke, Makiko

AU - Fujise, Masako

AU - Eto, Fujiko

AU - Harada, Yukiko

AU - Kadowaki, Masako

AU - Shimono, Nobuyuki

AU - Kang, Dongchon

PY - 2010/4/1

Y1 - 2010/4/1

N2 - Fluoroquinolone (FQ) resistance is usually caused by point mutations within the quinolone resistance-determining regions (QRDRs) of gyrA, gyrB, parC and/or parE. However, little is known about the worldwide increase in FQ-resistant Escherichia coli or, more specifically, about the geographical distribution of QRDR mutations and the clonal spread of isolates. In this study, we analysed 68 FQ-resistant E. coli isolates from eight Asian countries using QRDR amino acid mutation patterns and examined their susceptibility to FQs. Of the isolates, 38% had mutations at S83 and D87 of GyrA and S80 of ParC (MM/-/M-/-) and 34% had mutations at S83 and D87 of GyrA, S80 of ParC and S458 of ParE (MM/-/M-/M). MIC50 values (minimum inhibitory concentrations for 50% of the isolates) for isolates with at least mutation at S458 of ParE for ciprofloxacin and prulifloxacin were relatively higher than MIC50 values of isolates without this mutation. Based on their geographic distribution and the QRDR mutation patterns, the isolates were divided into a common type in which the organisms were isolated from three or more countries, and a local type in which the isolates were from one or two countries. Mutation types at S83L and D87N in GyrA and S80I in ParC with no or another site in the QRDR were the most frequent among the FQ-resistant isolates, especially among the common type. Gene typing indicated that isolates in the common type were not similar between countries. These data suggest that the increase in FQ-resistant E. coli strains is mainly generated by mutations in the QRDR in each geographical area rather than through intercontinental spread.

AB - Fluoroquinolone (FQ) resistance is usually caused by point mutations within the quinolone resistance-determining regions (QRDRs) of gyrA, gyrB, parC and/or parE. However, little is known about the worldwide increase in FQ-resistant Escherichia coli or, more specifically, about the geographical distribution of QRDR mutations and the clonal spread of isolates. In this study, we analysed 68 FQ-resistant E. coli isolates from eight Asian countries using QRDR amino acid mutation patterns and examined their susceptibility to FQs. Of the isolates, 38% had mutations at S83 and D87 of GyrA and S80 of ParC (MM/-/M-/-) and 34% had mutations at S83 and D87 of GyrA, S80 of ParC and S458 of ParE (MM/-/M-/M). MIC50 values (minimum inhibitory concentrations for 50% of the isolates) for isolates with at least mutation at S458 of ParE for ciprofloxacin and prulifloxacin were relatively higher than MIC50 values of isolates without this mutation. Based on their geographic distribution and the QRDR mutation patterns, the isolates were divided into a common type in which the organisms were isolated from three or more countries, and a local type in which the isolates were from one or two countries. Mutation types at S83L and D87N in GyrA and S80I in ParC with no or another site in the QRDR were the most frequent among the FQ-resistant isolates, especially among the common type. Gene typing indicated that isolates in the common type were not similar between countries. These data suggest that the increase in FQ-resistant E. coli strains is mainly generated by mutations in the QRDR in each geographical area rather than through intercontinental spread.

UR - http://www.scopus.com/inward/record.url?scp=76749107390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76749107390&partnerID=8YFLogxK

U2 - 10.1016/j.ijantimicag.2009.12.005

DO - 10.1016/j.ijantimicag.2009.12.005

M3 - Article

C2 - 20138480

AN - SCOPUS:76749107390

VL - 35

SP - 387

EP - 391

JO - International Journal of Antimicrobial Agents

JF - International Journal of Antimicrobial Agents

SN - 0924-8579

IS - 4

ER -