Abstract
We have analyzed the expression profiles of ten genes in terms of testis development and organ specificity in rat, which were selected from 215 round spermatid-specific transcripts listed in a database. Out of the ten genes, we directed our attention to one gene, a germ cell-less like-2 gene (gcl-2), a homolog of Drosophila gcl gene (gcl), which is a component of the germ plasma and required for primordial germ cell formation. Rat genome contains duplicate rat gcl-2 (rgcl-2) genes, rgcl-2A and rgcl-2B, both of which are located at Xq13. RT-PCR analysis showed that the expression of the two genes was up-regulated during testis development and that they were predominantly expressed in the testis. Both rgcl-2A and rgcl-2B encode a protein of 498 amino acid residues, showing 90.56% identity at the amino acid level. Confocal laser scanning microscopy revealed that rgcl-2 protein was synthesized in the cytoplasm of elongating spermatids and at least a part of it was integrated into the middle piece of spermatozoa during spermiogenesis. Immunogold electron microscopy uncovered that rgcl-2 was localized at the abaxial (convex) surface of outer dense fibers (ODF) of rat sperm flagella. Therefore, we concluded that rgcl-2 is a new component of ODF in sperm flagella.
| Original language | English |
|---|---|
| Pages (from-to) | 749-756 |
| Number of pages | 8 |
| Journal | Reproduction |
| Volume | 134 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 1 2007 |
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All Science Journal Classification (ASJC) codes
- Obstetrics and Gynaecology
- Cell Biology
- Endocrinology
- Embryology
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Germ cell-less like-2 protein is a new component of outer dense fibers in rat sperm flagella. / Murayama, Emi; Katoh, Michiko; Kanebayashi, Akina; Kaneko, Takane; Shibata, Yosaburo; Inai, Tetsuichiro; Iida, Hiroshi.
In: Reproduction, Vol. 134, No. 6, 01.12.2007, p. 749-756.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Germ cell-less like-2 protein is a new component of outer dense fibers in rat sperm flagella
AU - Murayama, Emi
AU - Katoh, Michiko
AU - Kanebayashi, Akina
AU - Kaneko, Takane
AU - Shibata, Yosaburo
AU - Inai, Tetsuichiro
AU - Iida, Hiroshi
PY - 2007/12/1
Y1 - 2007/12/1
N2 - We have analyzed the expression profiles of ten genes in terms of testis development and organ specificity in rat, which were selected from 215 round spermatid-specific transcripts listed in a database. Out of the ten genes, we directed our attention to one gene, a germ cell-less like-2 gene (gcl-2), a homolog of Drosophila gcl gene (gcl), which is a component of the germ plasma and required for primordial germ cell formation. Rat genome contains duplicate rat gcl-2 (rgcl-2) genes, rgcl-2A and rgcl-2B, both of which are located at Xq13. RT-PCR analysis showed that the expression of the two genes was up-regulated during testis development and that they were predominantly expressed in the testis. Both rgcl-2A and rgcl-2B encode a protein of 498 amino acid residues, showing 90.56% identity at the amino acid level. Confocal laser scanning microscopy revealed that rgcl-2 protein was synthesized in the cytoplasm of elongating spermatids and at least a part of it was integrated into the middle piece of spermatozoa during spermiogenesis. Immunogold electron microscopy uncovered that rgcl-2 was localized at the abaxial (convex) surface of outer dense fibers (ODF) of rat sperm flagella. Therefore, we concluded that rgcl-2 is a new component of ODF in sperm flagella.
AB - We have analyzed the expression profiles of ten genes in terms of testis development and organ specificity in rat, which were selected from 215 round spermatid-specific transcripts listed in a database. Out of the ten genes, we directed our attention to one gene, a germ cell-less like-2 gene (gcl-2), a homolog of Drosophila gcl gene (gcl), which is a component of the germ plasma and required for primordial germ cell formation. Rat genome contains duplicate rat gcl-2 (rgcl-2) genes, rgcl-2A and rgcl-2B, both of which are located at Xq13. RT-PCR analysis showed that the expression of the two genes was up-regulated during testis development and that they were predominantly expressed in the testis. Both rgcl-2A and rgcl-2B encode a protein of 498 amino acid residues, showing 90.56% identity at the amino acid level. Confocal laser scanning microscopy revealed that rgcl-2 protein was synthesized in the cytoplasm of elongating spermatids and at least a part of it was integrated into the middle piece of spermatozoa during spermiogenesis. Immunogold electron microscopy uncovered that rgcl-2 was localized at the abaxial (convex) surface of outer dense fibers (ODF) of rat sperm flagella. Therefore, we concluded that rgcl-2 is a new component of ODF in sperm flagella.
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UR - http://www.scopus.com/inward/citedby.url?scp=37549024528&partnerID=8YFLogxK
U2 - 10.1530/REP-07-0358
DO - 10.1530/REP-07-0358
M3 - Article
C2 - 18042632
AN - SCOPUS:37549024528
VL - 134
SP - 749
EP - 756
JO - Reproduction
JF - Reproduction
SN - 1470-1626
IS - 6
ER -