Ghrelin Acts at the Nucleus of the Solitary Tract to Decrease Arterial Pressure in Rats

Yingzi Lin, Kiyoshi Matsumura, Masayo Fukuhara, Shuntaro Kagiyama, Koji Fujii, Mitsuo Iida

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

Ghrelin is an orexigenic peptide originally isolated from the stomach. Intracerebroventricular administration of ghrelin has been shown to elicit decreases in arterial pressure and renal sympathetic nerve activity in conscious rabbits. The aim of the present study was to determine the role of ghrelin in the brain stem in cardiovascular responses in rats. Unilateral microinjection of ghrelin into the nucleus of the solitary tract significantly decreased the mean arterial pressure and heart rate (- 17.3±0.8 mm Hg and -13.6±3.5 bpm by 20 pmol). The microinjection of ghrelin into the nucleus of the solitary tract also suppressed the renal sympathetic nerve activity (-29.5±3.4%; P<0.0001). Pretreatment with intravenous injection of pentolinium (5 mg/kg), a ganglion-blocking agent, eliminated these cardiovascular responses induced by the microinjection of ghrelin (20 pmol) into the nucleus of the solitary tract; however, pretreatment with intravenous injection of atropine sulfate (0.1 mg/kg), an antagonist of muscarinic acetylcholine receptors, failed to prevent them. In contrast, unilateral microinjection of ghrelin into the area postrema, rostral, and caudal ventrolateral medulla caused no significant changes in the mean arterial pressure and heart rate. On the other hand, immunohistochemical study revealed that the receptor for ghrelin, the growth hormone secretagogue receptor, was expressed in the neuronal cells of the nucleus of the solitary tract and the dorsal motor nucleus of the vagus, but not in the cells of the area postrema. These results suggest that ghrelin acts at the nucleus of the solitary tract to suppress sympathetic activity and to decrease arterial pressure in rats.

Original languageEnglish
Pages (from-to)977-982
Number of pages6
JournalHypertension
Volume43
Issue number5
DOIs
Publication statusPublished - May 1 2004

Fingerprint

Ghrelin
Solitary Nucleus
Arterial Pressure
Microinjections
Ghrelin Receptor
Area Postrema
Intravenous Injections
Pentolinium Tartrate
Heart Rate
Kidney
Cardiovascular Agents
Muscarinic Receptors
Cell Nucleus
Atropine
Ganglia
Brain Stem
Stomach
Rabbits
Peptides

All Science Journal Classification (ASJC) codes

  • Internal Medicine

Cite this

Lin, Y., Matsumura, K., Fukuhara, M., Kagiyama, S., Fujii, K., & Iida, M. (2004). Ghrelin Acts at the Nucleus of the Solitary Tract to Decrease Arterial Pressure in Rats. Hypertension, 43(5), 977-982. https://doi.org/10.1161/01.HYP.0000122803.91559.55

Ghrelin Acts at the Nucleus of the Solitary Tract to Decrease Arterial Pressure in Rats. / Lin, Yingzi; Matsumura, Kiyoshi; Fukuhara, Masayo; Kagiyama, Shuntaro; Fujii, Koji; Iida, Mitsuo.

In: Hypertension, Vol. 43, No. 5, 01.05.2004, p. 977-982.

Research output: Contribution to journalArticle

Lin, Y, Matsumura, K, Fukuhara, M, Kagiyama, S, Fujii, K & Iida, M 2004, 'Ghrelin Acts at the Nucleus of the Solitary Tract to Decrease Arterial Pressure in Rats', Hypertension, vol. 43, no. 5, pp. 977-982. https://doi.org/10.1161/01.HYP.0000122803.91559.55
Lin, Yingzi ; Matsumura, Kiyoshi ; Fukuhara, Masayo ; Kagiyama, Shuntaro ; Fujii, Koji ; Iida, Mitsuo. / Ghrelin Acts at the Nucleus of the Solitary Tract to Decrease Arterial Pressure in Rats. In: Hypertension. 2004 ; Vol. 43, No. 5. pp. 977-982.
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