TY - JOUR
T1 - Ghrelin expression in islet cell tumors
T2 - Augmented expression of ghrelin in a case of glucagonoma with multiple endocrine neoplasm type I
AU - Iwakura, Hiroshi
AU - Hosoda, Kiminori
AU - Doi, Ryuichiro
AU - Komoto, Izumi
AU - Nishimura, Haruo
AU - Son, Choel
AU - Fujikura, Junji
AU - Tomita, Tsutomu
AU - Takaya, Kazuhiko
AU - Ogawa, Yoshihiro
AU - Hayashi, Tatsuya
AU - Inoue, Gen
AU - Akamizu, Takashi
AU - Hosoda, Hiroshi
AU - Kojima, Masayasu
AU - Kangawa, Kenji
AU - Imamura, Masayuki
AU - Nakao, Kazuwa
PY - 2002/11/1
Y1 - 2002/11/1
N2 - Ghrelin is a 28-amino acid peptide that regulates GH release together with GHRH and somatostatin. The expression of ghrelin has been detected in the stomach, small intestine, hypothalamus, pituitary gland, kidney, placenta, and testis. Recently it was reported that ghrelin is present in pancreatic α-cells and that it stimulates insulin secretion. In this study, we examined the ghrelin expression in two cases of glucagonoma and two cases of insulinoma by Northern blot analysis and immunohistochemistry. Ghrelin expression was identified in a case of glucagonoma associated with multiple endocrine neoplasm type I both by Northern blot analysis using total RNA and by immunohistochemistry, although the plasma ghrelin level was not elevated. This is the first case of tumor in which ghrelin gene expression was detected by Northern blot analysis using total RNA.
AB - Ghrelin is a 28-amino acid peptide that regulates GH release together with GHRH and somatostatin. The expression of ghrelin has been detected in the stomach, small intestine, hypothalamus, pituitary gland, kidney, placenta, and testis. Recently it was reported that ghrelin is present in pancreatic α-cells and that it stimulates insulin secretion. In this study, we examined the ghrelin expression in two cases of glucagonoma and two cases of insulinoma by Northern blot analysis and immunohistochemistry. Ghrelin expression was identified in a case of glucagonoma associated with multiple endocrine neoplasm type I both by Northern blot analysis using total RNA and by immunohistochemistry, although the plasma ghrelin level was not elevated. This is the first case of tumor in which ghrelin gene expression was detected by Northern blot analysis using total RNA.
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U2 - 10.1210/jc.2002-020882
DO - 10.1210/jc.2002-020882
M3 - Article
C2 - 12414844
AN - SCOPUS:18744391060
VL - 87
SP - 4885
EP - 4888
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
SN - 0021-972X
IS - 11
ER -
