Ght2+ is required for UDP-galactose synthesis from extracellular galactose by Schizosaccharomyces pombe

Tomohiko Matsuzawa, Futoshi Hara, Naotaka Tanaka, Hideki Tohda, Kaoru Takegawa

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Schizosaccharomyces pombe has eight hexose transporter genes, ght1 + to ght8 +. Here we report that ght2 +, which is highly expressed in the presence of glucose, is essential for UDP-galactose synthesis from extracellular galactose when cells grow on glucose. The galactosylation defect of a uge1Δ mutant defective in synthesis of UDP-galactose from glucose was suppressed in galactose-containing medium, but disruption of ght2 + in the uge1Δ mutant reversed suppression of the galactosylation defect. Expression of Saccharomyces cerevisiae GAL2 in uge1Δght2Δ cells suppressed the defective galactosylation phenotype in galactose-containing medium. These results indicate that galactose is transported from the medium to the cytosol in a Ght2-dependent manner, and is then converted into UDP-galactose.

Original languageEnglish
Pages (from-to)4957-4964
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume97
Issue number11
DOIs
Publication statusPublished - Jun 1 2013

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Ght2<sup>+</sup> is required for UDP-galactose synthesis from extracellular galactose by Schizosaccharomyces pombe'. Together they form a unique fingerprint.

Cite this