Global regulation by horizontally transferred regulators establishes the pathogenicity of escherichia coli

Hiroyuki Abe, Akira Miyahara, Taku Oshima, Kosuke Tashiro, Yoshitoshi Ogura, Satoru Kuhara, Naotake Ogasawara, Tetsuya Hayashi, Toru Tobe

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Enterohemorrhagic Escherichia coli is an emerging pathogen that causes diarrhea and hemolytic uremic syndrome. Much of the genomic information that affects virulence is acquired by horizontal transfer. Genes necessary for attaching and effacing lesions are located in the locus for enterocyte effacement (LEE) pathogenicity island. LEE gene transcription is positively regulated by Ler, which is also encoded by the LEE, and by Pch regulators, which are encoded at other loci. Here we identified genes whose transcription profiles were similar to those of the LEE genes, by comparing the effects of altering ler and pch transcript levels. We assigned these genes into two classes, according to their transcription profiles. By determining the binding profiles for Ler and Pch, we showed that both were involved in regulating one class of genes, but only Pch was involved in regulating the other class. Binding sites were found in the coding region as well as the promoter region of regulated genes, which include genes common to K12 strains as well as 0157-specific genes, suggesting that both act as a global regulator. These results indicate that Ler and Pch orchestrate the transcription of virulence genes, which are captured by horizontal transfer and scattered throughout the chromosome.

Original languageEnglish
Pages (from-to)25-38
Number of pages14
JournalDNA Research
Volume15
Issue number1
DOIs
Publication statusPublished - Dec 1 2008

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Virulence
Escherichia coli
Enterocytes
Genes
Enterohemorrhagic Escherichia coli
Genomic Islands
Hemolytic-Uremic Syndrome
Genetic Promoter Regions
Diarrhea
Chromosomes
Binding Sites

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Global regulation by horizontally transferred regulators establishes the pathogenicity of escherichia coli. / Abe, Hiroyuki; Miyahara, Akira; Oshima, Taku; Tashiro, Kosuke; Ogura, Yoshitoshi; Kuhara, Satoru; Ogasawara, Naotake; Hayashi, Tetsuya; Tobe, Toru.

In: DNA Research, Vol. 15, No. 1, 01.12.2008, p. 25-38.

Research output: Contribution to journalArticle

Abe, Hiroyuki ; Miyahara, Akira ; Oshima, Taku ; Tashiro, Kosuke ; Ogura, Yoshitoshi ; Kuhara, Satoru ; Ogasawara, Naotake ; Hayashi, Tetsuya ; Tobe, Toru. / Global regulation by horizontally transferred regulators establishes the pathogenicity of escherichia coli. In: DNA Research. 2008 ; Vol. 15, No. 1. pp. 25-38.
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