Glucose reduced PDGF production and cell proliferation of cultured vascular endothelial cells

F. Umeda, T. Yamauchi, N. Nakashima, H. Ono, H. Nawata, M. Masuko, K. Nakayama, A. Tatematsu

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8 Citations (Scopus)

Abstract

The effect of glucose on PDGF production and cell proliferation was studied on cultured bovine aortic endothelial cells. PDGF levels were measured using an enzyme-linked immunosorbent assay technique newly developed in our laboratory. The cell proliferation rate was determined on the basis of 3H-thymidine incorporation into cellular DNA. PDGF levels in culture medium were below the detection limit of the assay. However, PDGF levels were measurable in cultured endothelial cells at confluence. Both PDGF production and thymidine incorporation were significantly reduced in the endothelial cells cultured with high concentrations of glucose. These results suggest that reduced PDGF production and cell proliferation may be involved in altered vascular endothelial function in diabetics.

Original languageEnglish
Pages (from-to)274-277
Number of pages4
JournalHormone and Metabolic Research
Volume23
Issue number6
DOIs
Publication statusPublished - Jan 1 1991

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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