Glutamate acts as a key signal linking glucose metabolism to incretin/cAMP action to amplify insulin secretion

Ghupurjan Gheni, Masahito Ogura, Masahiro Iwasaki, Norihide Yokoi, Kohtaro Minami, Yasumune Nakayama, Kazuo Harada, Benoit Hastoy, Xichen Wu, Harumi Takahashi, Kazushi Kimura, Toshiya Matsubara, Ritsuko Hoshikawa, Naoya Hatano, Kenji Sugawara, Tadao Shibasaki, Nobuya Inagaki, Takeshi Bamba, Akira Mizoguchi, Eiichiro FukusakiPatrik Rorsman, Susumu Seino

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Incretins, hormones released by the gut after meal ingestion, are essential for maintaining systemic glucose homeostasis by stimulating insulin secretion. The effect of incretins on insulin secretion occurs only at elevated glucose concentrations and is mediated by cAMP signaling, but the mechanism linking glucose metabolism and cAMP action in insulin secretion is unknown. We show here, using a metabolomics-based approach, that cytosolic glutamate derived from the malate-aspartate shuttle upon glucose stimulation underlies the stimulatory effect of incretins and that glutamate uptake into insulin granules mediated by cAMP/ PKA signaling amplifies insulin release. Glutamate production is diminished in an incretin-unresponsive, insulin-secreting β cell line and pancreatic islets of animal models of human diabetes and obesity. Conversely, a membrane-permeable glutamate precursor restores amplification of insulin secretion in these models. Thus, cytosolic glutamate represents the elusive link between glucose metabolism and cAMP action in incretin-induced insulin secretion.

Original languageEnglish
Pages (from-to)661-673
Number of pages13
JournalCell Reports
Volume9
Issue number2
DOIs
Publication statusPublished - Jan 1 2014

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Incretins
Metabolism
Glutamic Acid
Insulin
Glucose
Metabolomics
Insulin-Secreting Cells
Islets of Langerhans
Aspartic Acid
Medical problems
Meals
Homeostasis
Animal Models
Amplification
Obesity
Eating
Hormones
Animals
Cells
Cell Line

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Gheni, G., Ogura, M., Iwasaki, M., Yokoi, N., Minami, K., Nakayama, Y., ... Seino, S. (2014). Glutamate acts as a key signal linking glucose metabolism to incretin/cAMP action to amplify insulin secretion. Cell Reports, 9(2), 661-673. https://doi.org/10.1016/j.celrep.2014.09.030

Glutamate acts as a key signal linking glucose metabolism to incretin/cAMP action to amplify insulin secretion. / Gheni, Ghupurjan; Ogura, Masahito; Iwasaki, Masahiro; Yokoi, Norihide; Minami, Kohtaro; Nakayama, Yasumune; Harada, Kazuo; Hastoy, Benoit; Wu, Xichen; Takahashi, Harumi; Kimura, Kazushi; Matsubara, Toshiya; Hoshikawa, Ritsuko; Hatano, Naoya; Sugawara, Kenji; Shibasaki, Tadao; Inagaki, Nobuya; Bamba, Takeshi; Mizoguchi, Akira; Fukusaki, Eiichiro; Rorsman, Patrik; Seino, Susumu.

In: Cell Reports, Vol. 9, No. 2, 01.01.2014, p. 661-673.

Research output: Contribution to journalArticle

Gheni, G, Ogura, M, Iwasaki, M, Yokoi, N, Minami, K, Nakayama, Y, Harada, K, Hastoy, B, Wu, X, Takahashi, H, Kimura, K, Matsubara, T, Hoshikawa, R, Hatano, N, Sugawara, K, Shibasaki, T, Inagaki, N, Bamba, T, Mizoguchi, A, Fukusaki, E, Rorsman, P & Seino, S 2014, 'Glutamate acts as a key signal linking glucose metabolism to incretin/cAMP action to amplify insulin secretion', Cell Reports, vol. 9, no. 2, pp. 661-673. https://doi.org/10.1016/j.celrep.2014.09.030
Gheni, Ghupurjan ; Ogura, Masahito ; Iwasaki, Masahiro ; Yokoi, Norihide ; Minami, Kohtaro ; Nakayama, Yasumune ; Harada, Kazuo ; Hastoy, Benoit ; Wu, Xichen ; Takahashi, Harumi ; Kimura, Kazushi ; Matsubara, Toshiya ; Hoshikawa, Ritsuko ; Hatano, Naoya ; Sugawara, Kenji ; Shibasaki, Tadao ; Inagaki, Nobuya ; Bamba, Takeshi ; Mizoguchi, Akira ; Fukusaki, Eiichiro ; Rorsman, Patrik ; Seino, Susumu. / Glutamate acts as a key signal linking glucose metabolism to incretin/cAMP action to amplify insulin secretion. In: Cell Reports. 2014 ; Vol. 9, No. 2. pp. 661-673.
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AU - Shibasaki, Tadao

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AU - Bamba, Takeshi

AU - Mizoguchi, Akira

AU - Fukusaki, Eiichiro

AU - Rorsman, Patrik

AU - Seino, Susumu

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