ATP evoked an increase in intracellular Ca2+ concentration ([Ca]i) in hippocampal neurones in the presence of tetrodotoxin (TTX, 3 μM), hexamethonium (C6,100 μM), D-2-amino-phosphonovalerate (APV, 100 μM), 6- cyano-7-nitro quinoxalline-2,3-dione (CNQX, 30 μM), bicuculline (10 μM) and cadmium (Cd2+, 300 μM). The increase was blocked by suramin (100 fiM), a P2-purinocep- tor blocker. These results suggest that ATP evokes an increase in [Ca]; through P2-purinoceptor. As a result of measuring [Ca], in multiple cells simultaneously, it was suggested that glutamatergic neurones connect with purinergic neurones and glutamate (10 /xM) stimulated the release of ATP causing an increase in [Ca]; in postsyn- aptic neurones.
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