TY - JOUR
T1 - Glutamate-evoked release of adenosine 5’ -triphosphate causing an increase in intracellular calcium in hippocampal neurons
AU - Lnoue, Kazuhide
AU - Koizumi, Schuichi
AU - Nakazawa, Ken
PY - 1995/2
Y1 - 1995/2
N2 - ATP evoked an increase in intracellular Ca2+ concentration ([Ca]i) in hippocampal neurones in the presence of tetrodotoxin (TTX, 3 μM), hexamethonium (C6,100 μM), D-2-amino-phosphonovalerate (APV, 100 μM), 6- cyano-7-nitro quinoxalline-2,3-dione (CNQX, 30 μM), bicuculline (10 μM) and cadmium (Cd2+, 300 μM). The increase was blocked by suramin (100 fiM), a P2-purinocep- tor blocker. These results suggest that ATP evokes an increase in [Ca]; through P2-purinoceptor. As a result of measuring [Ca], in multiple cells simultaneously, it was suggested that glutamatergic neurones connect with purinergic neurones and glutamate (10 /xM) stimulated the release of ATP causing an increase in [Ca]; in postsyn- aptic neurones.
AB - ATP evoked an increase in intracellular Ca2+ concentration ([Ca]i) in hippocampal neurones in the presence of tetrodotoxin (TTX, 3 μM), hexamethonium (C6,100 μM), D-2-amino-phosphonovalerate (APV, 100 μM), 6- cyano-7-nitro quinoxalline-2,3-dione (CNQX, 30 μM), bicuculline (10 μM) and cadmium (Cd2+, 300 μM). The increase was blocked by suramin (100 fiM), a P2-purinocep- tor blocker. These results suggest that ATP evokes an increase in [Ca]; through P2-purinoceptor. As a result of measuring [Ca], in multiple cells simultaneously, it was suggested that glutamatergic neurones connect with purinergic neurones and glutamate (10 /xM) stimulated the release of ATP causing an increase in [Ca]; in postsyn- aptic neurones.
UR - http://www.scopus.com/inward/record.url?scp=0028838090&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028838090&partnerID=8YFLogxK
U2 - 10.1097/00001756-199502000-00008
DO - 10.1097/00001756-199502000-00008
M3 - Article
C2 - 7766838
AN - SCOPUS:0028838090
VL - 6
SP - 437
EP - 440
JO - NeuroReport
JF - NeuroReport
SN - 0959-4965
IS - 3
ER -