Glutamate release from astrocyte cell-line GL261 via alterations in the intracellular ion environment

Kenji Ono, Hiromi Suzuki, Madoka Higa, Kaori Tabata, Makoto Sawada

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Astrocytes modify and maintain neural activity and functions via gliotransmitter release such as, glutamate. They also change their properties and functions in response to alterations of ion environment resulting from neurotransmission; however, the direct evidence for whether intracellular ion alteration in astrocytes triggers gliotransmitter release is not indicated. Recent studies have reported that channelrhodopsin-2 (ChR2) is useful for alteration of intracellular ion environment in several types of cells with blue light exposure. Here, we show that ChR2-expressing GL261 (GLChR2) cells, clonal astrocytes, change their properties by photo-activation. Increased intracellular sodium and calcium ion concentrations and an altered membrane potential were observed in GLChR2 cells with blue light exposure. Alterations in the intracellular ion environment caused intracellular acidification and the inhibition of proliferation. In addition, it triggered glutamate release from GLChR2 cells. Glutamate from GLChR2 cells acted on N18 cells, clonal neuronal cells, as both a transmitter and neurotoxin depending on photo-activation. Our results show that the properties of ChR2-expressing astrocytes can be controlled by blue light exposure, and cation influx through photo-activated ChR2 might trigger functional cation influx via endogenous channels and result in the increase of glutamate release. Further, our results suggest that ChR2-expressing glial cells could become a useful tool in understanding the roles of glial cell activation and neural communication in the regulation of brain functions.

Original languageEnglish
Pages (from-to)245-257
Number of pages13
JournalJournal of Neural Transmission
Volume121
Issue number3
DOIs
Publication statusPublished - Mar 1 2014

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Astrocytes
Glutamic Acid
Ions
Cell Line
Light
Neuroglia
Cations
Neurotoxins
Synaptic Transmission
Membrane Potentials
Sodium
Calcium
Brain

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Psychiatry and Mental health
  • Biological Psychiatry

Cite this

Glutamate release from astrocyte cell-line GL261 via alterations in the intracellular ion environment. / Ono, Kenji; Suzuki, Hiromi; Higa, Madoka; Tabata, Kaori; Sawada, Makoto.

In: Journal of Neural Transmission, Vol. 121, No. 3, 01.03.2014, p. 245-257.

Research output: Contribution to journalArticle

Ono, Kenji ; Suzuki, Hiromi ; Higa, Madoka ; Tabata, Kaori ; Sawada, Makoto. / Glutamate release from astrocyte cell-line GL261 via alterations in the intracellular ion environment. In: Journal of Neural Transmission. 2014 ; Vol. 121, No. 3. pp. 245-257.
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