Glyceraldehyde-3-phosphate dehydrogenase mRNA expression in hepatocellular carcinoma

Motoyuki Yamagata, Masaki Mori, Nasim A. Begum, Kenji Shibuta, Katsuhiro Shimoda, Graham F. Barnard

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Glyceraldehyde-3-phosphate dehydrogenase (G3PDH) is often used as a control gene for mRNA expression, however it has been proposed to be overexpressed in all hepatocellular carcinomas (HCC). Equal amounts of tumor and paired normal (T/N) RNA, based on OD(260/280) nm, were compared using ethidium bromide staining, poly-T probing, gene-specific dot blot and Northern blots using control probes G3PDH, actin and histone H4. Using mRNA blots 13/20 surgical HCC pairs did not overexpress G3PDH. Those 7/20 intact samples which did appear to overexpress G3PDH on Northern blot could not be detected by poly-T probing of dot blots. The apparent overexpression was not specific for the control gene G3PDH nor for the malignancy HCC. It may represent partial mRNA degradation, or the presence of as yet unknown substances which interfere with absorption at 260/280 nm. We advise caution in selecting human T/N pairs for differential gene expression studies. For HCC, no clinicopathological variables, including cirrhosis, predicted whether a T/N sample pair was likely to be balanced or not.

Original languageEnglish
Pages (from-to)677-683
Number of pages7
JournalInternational journal of oncology
Volume12
Issue number3
DOIs
Publication statusPublished - Mar 1998

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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