Glycine-insensitive desensitization of N-methyl-d-aspartate receptors in acutely isolated mammalian central neurons

T. Shirasaki, T. Nakagawa, M. Wakamori, N. Tateishi, A. Fukuda, K. Murase, N. Akaike

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Acutely isolated rat central neurons were recorded by whole-cell voltage-clamp and responses to a class of excitatory amino acid N-methyl-d-aspartate (NMDA) were examined. Rapid application of NMDA evoked inward current consisted of a fast initial peak followed by a sustained component. Glycine potentiated both initial and desensitized states of the NMDA response with identical concentration-dependence. The initial response, but not the sustained component, was abolished when low concentration of NMDA was pre-applied, and glycine could not reverse the desensitization. This evidence suggests that the NMDA receptor desensitization is sensitive to NMDA but not to glycine, and support the hypothesis that glycine initiates the activation of NMDA receptors rather than that glycine prevents desensitization at NMDA receptors in these cells.

Original languageEnglish
Pages (from-to)93-98
Number of pages6
JournalNeuroscience Letters
Volume108
Issue number1-2
DOIs
Publication statusPublished - Jan 1 1990
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Glycine-insensitive desensitization of N-methyl-d-aspartate receptors in acutely isolated mammalian central neurons'. Together they form a unique fingerprint.

Cite this